Establishment and Characterization of OFT and OFO Cell Lines from Olive Flounder (Paralichthys olivaceus) for Use as Feeder Cells.

IF 3.6 3区 生物学 Q1 BIOLOGY
Ja Young Jo, Ju-Won Kim, Eun Soo Noh, Yong-Ok Kim, Seung Pyo Gong, Hee Jeong Kong, Jae Hoon Choi
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引用次数: 0

Abstract

Olive flounder (Paralichthys olivaceus) is a commercially important fish species in Japan, China, and the Republic of Korea. Despite numerous attempts to improve productivity, there have been no studies of in vitro germline stem cell (GSC) culture in this species. Here, olive flounder testicular and ovarian cell lines (OFT and OFO, respectively) were established and characterized. RT-PCR demonstrated that OFT and OFO expressed several gonadal somatic cell markers, including wt1 and fgf2, but lacked expression of germ cell markers, such as vasa, nanos2, and scp3. In addition, SNP analysis revealed that OFT originated from XY male P. olivaceus and OFO originated from XX female P. olivaceus. These results suggest that OFT was composed of Sertoli cells and OFO was composed of granulosa cells and theca cells. Finally, coculture of OFT or OFO with enriched male P. olivaceus GSCs isolated from the top 20% and 20-30% Percoll density gradient layers showed that GSCs were attached on both cell lines. In conclusion, we established P. olivaceus testicular and ovarian cell lines, which were expected to use for development of an in vitro GSC culture system.

橄榄比目鱼(palichthys olivaceus)供体细胞OFT和OFO细胞系的建立和鉴定。
橄榄比目鱼(学名:橄榄比目鱼)在日本、中国和韩国是一种重要的商业鱼类。尽管有许多提高生产力的尝试,还没有研究体外种系干细胞(GSC)培养在这个物种。本文建立了橄榄比目鱼睾丸细胞系和卵巢细胞系(分别为OFT和OFO),并对其进行了鉴定。RT-PCR显示,OFT和OFO表达了几种性腺体细胞标记物,包括wt1和fgf2,但缺乏生殖细胞标记物,如vasa、nanos2和scp3的表达。此外,SNP分析显示OFT来源于XY雄性P. olivaceus, OFO来源于XX雌性P. olivaceus。这些结果表明,OFO由支持细胞组成,OFO由颗粒细胞和鞘膜细胞组成。最后,将OFT或OFO与从前20% Percoll密度梯度层和20-30% Percoll密度梯度层分离的富集雄性橄榄虾GSCs共培养,结果表明,两种细胞系都附着了GSCs。总之,我们建立了橄榄虾睾丸和卵巢细胞系,有望用于体外培养系统的开发。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biology-Basel
Biology-Basel Biological Science-Biological Science
CiteScore
5.70
自引率
4.80%
发文量
1618
审稿时长
11 weeks
期刊介绍: Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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