Integration of Fluorescence Spectroscopy into a Photobioreactor for the Monitoring of Cyanobacteria.

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL
Borja García García, María Gabriela Fernández-Manteca, Celia Gómez-Galdós, Susana Deus Álvarez, Agustín P Monteoliva, José Miguel López-Higuera, José Francisco Algorri, Alain A Ocampo-Sosa, Luis Rodríguez-Cobo, Adolfo Cobo
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引用次数: 0

Abstract

Phytoplankton are essential to aquatic ecosystems but can cause harmful algal blooms (HABs) that threaten water quality, aquatic life, and human health. Developing new devices based on spectroscopic techniques offers a promising alternative for rapid and accurate monitoring of aquatic environments. However, phytoplankton undergo various physiological changes throughout their life cycle, leading to alterations in their optical properties, such as autofluorescence. In this study, we present a modification of a low-cost photobioreactor designed to implement fluorescence spectroscopy to analyze the evolution of spectral signals during phytoplankton growth cycles. This device primarily facilitates the characterization of changes in autofluorescence, providing valuable information for the development of future spectroscopic techniques for detecting and monitoring phytoplankton. Additionally, real-time testing was performed on cyanobacterial cultures, where changes in autofluorescence were observed under different conditions. This work demonstrates a cost-effective implementation of spectroscopic techniques within a photobioreactor, offering a preliminary analysis for the future development of functional field devices for monitoring aquatic ecosystems.

浮游植物对水生生态系统至关重要,但也可能导致有害藻华(HAB),威胁水质、水生生物和人类健康。开发基于光谱技术的新设备为快速、准确地监测水生环境提供了一种可行的替代方法。然而,浮游植物在整个生命周期中会发生各种生理变化,从而导致其光学特性发生改变,例如自发荧光。在本研究中,我们介绍了一种低成本光生物反应器的改良装置,旨在采用荧光光谱技术分析浮游植物生长周期中光谱信号的演变。该装置主要有助于描述自发荧光的变化,为未来开发用于检测和监测浮游植物的光谱技术提供宝贵信息。此外,还对蓝藻培养物进行了实时测试,在不同条件下观察到自发荧光的变化。这项工作展示了在光生物反应器内实施光谱技术的成本效益,为今后开发用于监测水生生态系统的功能性现场设备提供了初步分析。
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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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