Novel anticoagulant-preservative solution maintained the hemostatic function of cold stored whole blood for 56 days.

IF 2.5 3区医学 Q2 HEMATOLOGY

Transfusion Pub Date : 2025-03-25 DOI:10.1111/trf.18207

K M Reddoch-Cardenas, J A Cancelas, S Nestheide, N Rugg, K Peña, C S McIntosh, J Ferdin, J Talackine, J Parker, L A Jensen, R Gonzales, J R Hess, M Zia

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引用次数: 0

Abstract

Background: Whole blood (WB) is an efficient product for field medical resuscitation because of its unitary composition, tolerance for storage on ice and in field refrigerators, and simplicity of use. We measured quality parameters of a novel 8-week WB storage system.

Study design and methods: Here, 500 mL of WB from healthy donors was collected in 70 mL of CPDA-1, leukoreduced with a platelet-sparing filter, pooled into ABO-compatible two-unit pools, and split into matched pairs of equal volume designated as Test or Control units. Test units received an additional 50 mL of a novel WB preservative solution (APEX units, Hemerus Medical, St Paul, MN). A total of 15 paired WB units were evaluated at Day 0 (D0) and periodically up to Day 56 (D56) of storage at 1-6°C across two centers. Quality testing included cellularity, ATP concentrations, hemolysis, blood gases, metabolites, coagulation factor levels, thromboelastography (TEG), and bacterial culture.

Results: At D56, APEX units displayed higher RBC ATP concentration (3.14 vs. 2.18 μmol/gHb, p = 0.001), pH (6.53 vs. 6.50, p = 0.01), and higher bicarbonate reserve (8 vs. 5.4, p < 0.0001). D56 APEX units had greater platelet contribution to TEG clot strength (p < 0.01) and better preservation of red cell ATP (p < 0.001). Activities of fibrinogen, factor VIII, factor V, and protein S activity in APEX units remained within the reference levels on D56. No bacterial contamination was detected at the end of storage.

Discussion: These findings suggest that APEX preserves RBCs effectively and maintains platelet and plasma coagulation functions for up to 56 days.

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新型抗凝保鲜液可使冷藏全血的止血功能维持56天。

背景：全血（WB）是一种有效的战地医疗复苏产品，因为它的单一成分，耐受性在冰上和野外冰箱中储存，并且使用简单。我们测量了一种新型8周WB存储系统的质量参数。研究设计和方法：在这里，从健康供者收集500 mL白细胞白蛋白，用70 mL CPDA-1，用血小板保留过滤器进行白细胞诱导，汇集到abo兼容的双单位池中，并分成匹配的等体积成对，指定为测试或控制单位。测试单位接受额外的50ml新型WB防腐剂溶液（APEX单位，Hemerus Medical, St Paul， MN）。共有15个配对的WB单位在第0天（D0）进行评估，并定期在两个中心的1-6°C储存至第56天（D56）。质量检测包括细胞结构、ATP浓度、溶血、血气、代谢物、凝血因子水平、血栓弹性成像（TEG）和细菌培养。结果：在D56时，APEX单元显示出更高的红细胞ATP浓度（3.14 vs. 2.18 μmol/gHb, p = 0.001）， pH （6.53 vs. 6.50, p = 0.01）和更高的碳酸氢盐储备（8 vs. 5.4, p）。这些发现表明APEX可有效保存红细胞，维持血小板和血浆凝血功能长达56天。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transfusion 医学-血液学

CiteScore

4.70

自引率

20.70%

发文量

426

审稿时长

1 months

期刊介绍： TRANSFUSION is the foremost publication in the world for new information regarding transfusion medicine. Written by and for members of AABB and other health-care workers, TRANSFUSION reports on the latest technical advances, discusses opposing viewpoints regarding controversial issues, and presents key conference proceedings. In addition to blood banking and transfusion medicine topics, TRANSFUSION presents submissions concerning patient blood management, tissue transplantation and hematopoietic, cellular, and gene therapies.