Single-cell parallel plate mechanics by side-view optical microscopy-assisted atomic force microscopy.

IF 4.6 3区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Mingyang Yang, Yanqi Yang, Lianqing Liu, Mi Li
{"title":"Single-cell parallel plate mechanics by side-view optical microscopy-assisted atomic force microscopy.","authors":"Mingyang Yang, Yanqi Yang, Lianqing Liu, Mi Li","doi":"10.1039/d5na00012b","DOIUrl":null,"url":null,"abstract":"<p><p>The mechanical forces of individual cells are crucial for physiological and pathological processes, and particularly atomic force microscopy (AFM)-based force spectroscopy has become an important and widely used tool for single-cell mechanical measurements. Here, we present a single-cell parallel plate mechanical measurement method based on side-view optical microscopy-assisted AFM by using a wedged probe, which enables visualization of the uniaxial AFM force spectroscopy process of the cell in real time from the vertical cross-sectional view. With this method, AFM whole-cell compression assays with uniaxial loading forces were performed to quantify the Young's modulus of the entire cell, and the effects of experimental parameters (ramp rate of the AFM probe) and drugs (actin inhibitors) on the measured cell Young's modulus were examined. Additionally, by attaching a living cell to the parallel plate of the wedged probe, AFM-based uniaxial single-cell force spectroscopy (SCFS) assays were performed to measure the adhesion forces of individual cells. The study illustrates a promising approach for single-cell mechanical analysis, which will benefit the application of AFM-based force spectroscopy in the field of mechanobiology to reveal additional insights into the regulatory role of mechanical cues in life activities.</p>","PeriodicalId":18806,"journal":{"name":"Nanoscale Advances","volume":" ","pages":""},"PeriodicalIF":4.6000,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11931428/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanoscale Advances","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1039/d5na00012b","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

Abstract

The mechanical forces of individual cells are crucial for physiological and pathological processes, and particularly atomic force microscopy (AFM)-based force spectroscopy has become an important and widely used tool for single-cell mechanical measurements. Here, we present a single-cell parallel plate mechanical measurement method based on side-view optical microscopy-assisted AFM by using a wedged probe, which enables visualization of the uniaxial AFM force spectroscopy process of the cell in real time from the vertical cross-sectional view. With this method, AFM whole-cell compression assays with uniaxial loading forces were performed to quantify the Young's modulus of the entire cell, and the effects of experimental parameters (ramp rate of the AFM probe) and drugs (actin inhibitors) on the measured cell Young's modulus were examined. Additionally, by attaching a living cell to the parallel plate of the wedged probe, AFM-based uniaxial single-cell force spectroscopy (SCFS) assays were performed to measure the adhesion forces of individual cells. The study illustrates a promising approach for single-cell mechanical analysis, which will benefit the application of AFM-based force spectroscopy in the field of mechanobiology to reveal additional insights into the regulatory role of mechanical cues in life activities.

求助全文
约1分钟内获得全文 求助全文
来源期刊
Nanoscale Advances
Nanoscale Advances Multiple-
CiteScore
8.00
自引率
2.10%
发文量
461
审稿时长
9 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信