Enhancing the Performance of Tangential Flow Microfiltration for Bioreactor Clarification.

IF 3.3 4区 工程技术 Q2 CHEMISTRY, PHYSICAL
Amir Hossein Mostafavi, Liang-Kai Chu, Xianghong Qian, John Paul Smelko, Da Zhang, Andrew Zydney, Sumith Ranil Wickramasinghe
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Abstract

Tangential flow microfiltration is easily adapted for batch and continuous bioreactor clarification. The permeate can be introduced directly to the subsequent capture step. However, the commercial use of tangential flow filtration (TFF) is limited by membrane fouling, leading to a compromised performance. Here, we explored the possibility of reducing membrane fouling by integrating a hydrocyclone as the primary clarification operation. The overflow from the hydrocyclone was introduced directly as the feed to the microfiltration module. Chinese hamster ovary cells were used as the feed stream to investigate the feasibility of this integrated process. A range of cell viabilities from 0% (cell lysate) to 96% were investigated. The cell densities ranged from 0.9 to 10 million cells per mL. Two commercially available hollow fiber microfiltration membranes were used, an essentially symmetric membrane and a reverse asymmetric membrane where the more open support structure faced the feed stream. The reverse asymmetric membrane was more resistant to fouling in the absence of an integrated hydrocyclone. Integrating a hydrocyclone led to a reduction in the flux decline for the symmetric membrane, but did not affect the performance of the reverse asymmetric membrane. The careful choice of membrane morphology and pore size is important when designing an integrated process.

提高切向流微滤在生物反应器澄清中的性能。
切向流微滤很容易适应间歇式和连续式生物反应器澄清。渗透液可以直接引入到后续的捕获步骤。然而,切向流过滤(TFF)的商业应用受到膜污染的限制,导致性能下降。在这里,我们探讨了通过集成水力旋流器作为主要澄清操作来减少膜污染的可能性。从水力旋流器流出的溢流直接作为进料引入微滤模块。以中国仓鼠卵巢细胞为饲料源,探讨了这一整合过程的可行性。细胞存活率从0%(细胞裂解物)到96%进行了研究。细胞密度从每毫升0.9到1000万个细胞不等。使用了两种市售的中空纤维微滤膜,一种是基本对称的膜,另一种是反不对称的膜,其中更开放的支撑结构面向饲料流。在没有一体化水力旋流器的情况下,反不对称膜具有更强的抗污染能力。集成水力旋流器使对称膜的通量下降幅度减小,但不影响反不对称膜的性能。在设计集成工艺时,仔细选择膜形态和孔径是很重要的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Membranes
Membranes Chemical Engineering-Filtration and Separation
CiteScore
6.10
自引率
16.70%
发文量
1071
审稿时长
11 weeks
期刊介绍: Membranes (ISSN 2077-0375) is an international, peer-reviewed open access journal of separation science and technology. It publishes reviews, research articles, communications and technical notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided.
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