The Antifungal Activity of a Polygalacturonic and Caprylic Acid Ointment in an In Vitro, Three-Dimensional Wound Biofilm Model.

IF 4.2 2区 生物学 Q2 MICROBIOLOGY
Bahgat Z Gerges, Joel Rosenblatt, Y-Lan Truong, Ying Jiang, Issam I Raad
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Abstract

Candida colonization and biofilms are significant contributors to impaired wound healing. Consequently, improved treatments are needed to eradicate Candida biofilms in wounds. Wounds present complex biofilm extracellular matrix environments, with microbial cells frequently enmeshed in matrices comprising wound exudate macromolecular gels. We evaluated the ability of a polygalacturonic and caprylic acid (PG + CAP) ointment to eradicate Candida albicans, C. parapsilosis, C. glabrata, C. tropicalis, and C. auris biofilms in a fibrin gel wound biofilm model of the complex wound biofilm environment. Hypochlorous acid (HOCl) is a disinfecting antimicrobial agent that is widely used as wound irrigant, and this was used as a comparator. A single treatment with PG + CAP reduced the number of viable organisms in the C. albicans and C. glabrata biofilms by over 5 log10, in the C. parapsilosis and C. auris biofilms by over 4 log10, and in the C. tropicalis biofilm by 3.85 log10. PG + CAP was superior (p < 0.01) to HOCl in eradicating all Candida species biofilms, except for C. auris, for which both treatments fully eradicated all viable organisms. The use of HOCl in Candida-colonized wounds should include consideration of the extracellular matrix load in the wound bed. PG + CAP warrants further study in wounds compromised by Candida biofilms.

聚半乳糖醛酸辛酸软膏在体外三维伤口生物膜模型中的抗真菌活性。
念珠菌定植和生物膜是伤口愈合受损的重要因素。因此,需要改进治疗方法来根除伤口中的念珠菌生物膜。伤口呈现复杂的生物膜细胞外基质环境,微生物细胞经常被包裹在伤口渗出物大分子凝胶的基质中。在复杂伤口生物膜环境的纤维蛋白凝胶伤口生物膜模型中,我们评估了聚半乳糖醛酸和丙烯酸(PG + CAP)软膏根除白色念珠菌、副枯枝假丝酵母、光秃假丝酵母、热带假丝酵母和耳假丝酵母生物膜的能力。次氯酸(HOCl)是一种广泛用于伤口冲洗的消毒抗菌剂,并作为比较物。单次PG + CAP处理可使白色念珠菌和光秃秃念珠菌生物膜中的活菌数量减少5 log10以上,使副枯枝念珠菌和耳念珠菌生物膜中的活菌数量减少4 log10以上,使热带念珠菌生物膜中的活菌数量减少3.85 log10以上。PG + CAP对所有念珠菌生物膜的杀灭效果均优于HOCl (p < 0.01),但对金黄色念珠菌的杀灭效果均优于HOCl (p < 0.01)。在假丝酵母菌定植的伤口中使用HOCl应考虑到伤口床的细胞外基质负荷。PG + CAP在假丝酵母生物膜损伤的伤口中值得进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Fungi
Journal of Fungi Medicine-Microbiology (medical)
CiteScore
6.70
自引率
14.90%
发文量
1151
审稿时长
11 weeks
期刊介绍: Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.
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