Molluscicidal Screening of Hypocreales Fungi from a Brazilian Cerrado Cave Against Biomphalaria glabrata Snails.

IF 4.2 2区生物学 Q2 MICROBIOLOGY

Journal of Fungi Pub Date : 2025-02-21 DOI:10.3390/jof11030173

Dominnyke Slater Santos Neves, Cyntia Ayumi Yokota Harayashiki, Pedro Henrique Félix de Oliveira, Thiago Lopes Rocha, Jadson Diogo Pereira Bezerra

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引用次数: 0

Abstract

Fungi play vital roles in ecosystems through parasitism, commensalism, and mutualism. Additionally, they are widely used in industry as bioactive compound producers and biological control agents. Biomphalaria glabrata is a freshwater snail often controlled with chemical molluscicides. However, developing effective alternatives to these chemical treatments is essential. This study evaluated the molluscicidal potential of culture supernatant from Hypocreales fungi isolated from a cave in the Brazilian Cerrado against the B. glabrata. The isolates were identified based on morphological features and ITS rDNA sequences. Fifteen filtrates of Hypocreales fungi were obtained and tested both pure and in different dilutions (10% and 50%) against newly hatched snails during 96 h of exposure. The fungal isolates were identified as belonging to the genera of Clonostachys (1), Cylindrocladiella (1), Fusarium (1), Gliocladiopsis (1), Keithomyces (1), Marquandomyces (1), Ovicillium (1), Pochonia (1), Purpureocillium (1), Sarcopodium (1), Sarocladium (1), Trichoderma (3), and Volutella (1). The results showed 93.33% (14) of the fungal filtrates induced significant mortality, indicating their molluscicidal activity, with Pochonia chlamydosporia FCCUFG 100 and Volutella aeria FCCUFG 107 causing 100% mortality in all dilutions. These results reveal the potential of Hypocreales fungi from a Brazilian Cerrado cave as a promising approach for snail control.

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巴西Cerrado洞穴下creales真菌对光螺生物phalaria glabrata蜗牛的杀螺筛选。

真菌通过寄生、共生和互惠在生态系统中起着至关重要的作用。此外，它们还广泛用于工业中作为生物活性化合物的生产者和生物防治剂。光螺（Biomphalaria glabrata）是一种淡水蜗牛，通常用化学杀螺剂控制。然而，开发这些化学处理的有效替代品是至关重要的。本研究评价了从巴西塞拉多的一个洞穴中分离出的Hypocreales真菌培养上清液对光斑贝虫的杀灭潜力。根据其形态特征和ITS rDNA序列进行鉴定。在96小时的暴露时间内，获得了15个Hypocreales真菌滤液，并对其进行了纯度和不同稀释度（10%和50%）对新孵化的蜗牛的检测。分离得到的真菌分别属于Clonostachys(1)、cydrocladiella(1)、Fusarium(1)、Gliocladiopsis(1)、Keithomyces(1)、Marquandomyces(1)、Ovicillium(1)、Pochonia(1)、Purpureocillium(1)、Sarcopodium(1)、Sarocladium(1)、Trichoderma(3)和Volutella(1)。结果表明，93.33%（14）的真菌滤出液具有显著的死亡率，表明其具有一定的杀软体活性。衣孢波胞菌FCCUFG 100和灰毒弧菌FCCUFG 107在所有稀释度下死亡率均为100%。这些结果揭示了从巴西塞拉多洞穴中发现的Hypocreales真菌作为一种有前途的蜗牛控制方法的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Fungi Medicine-Microbiology (medical)

CiteScore

6.70

自引率

14.90%

发文量

1151

审稿时长

11 weeks

期刊介绍： Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.