Antimicrobial efficacy of extracts obtained from aerial part, leaves, and flowers of Sphaeranthus indicus on different periodontal pathogens: An in vitro study.

Q2 Dentistry
Mahendra Rawaji Gawade, Amit Arvind Agrawal, Meenakshi Sreeram, Mahalaxmi Mohan
{"title":"Antimicrobial efficacy of extracts obtained from aerial part, leaves, and flowers of <i>Sphaeranthus indicus</i> on different periodontal pathogens: An <i>in vitro</i> study.","authors":"Mahendra Rawaji Gawade, Amit Arvind Agrawal, Meenakshi Sreeram, Mahalaxmi Mohan","doi":"10.4103/jisp.jisp_397_23","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong><i>Sphaeranthus indicus</i> is a medicinal plant widely used in the Indian traditional system of medicine for curing various ailments. However, there has been no report of any study testing its antimicrobial action against dental or periodontal pathogens. Hence, the aim of this study was to evaluate the <i>in vitro</i> antimicrobial activity of extract of whole plant, leaves, and flowers of <i>S</i>. <i>indicus</i> against four periodontal pathogens, namely, <i>Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans.</i></p><p><strong>Materials and methods: </strong>Extracts of the aerial part (whole plant), leaves, and flowers of <i>Sphaeranthus indicus</i> were prepared using cold maceration and ethanol extraction. The extracts were tested for their antimicrobial activity against periodontal pathogens by using <i>in vitro</i> serial dilution method at concentration of 12.5, 25, 50, and 100 μg/ml. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of all three groups were determined against all four periodontal pathogenic using thioglycollate broth and chlorhexidine as a positive control.</p><p><strong>Results: </strong><i>P</i>. <i>gingivalis</i> and <i>Fusobacterium nucleatum</i> were most susceptible to leaves extract (12.5 μg/ml and 25 μg/ml) followed by whole aerial plant extract (25 μg/ml and 50 μg/ml), but were least susceptible to flower extract (100 μg/ml). Another pathogen, <i>T</i>. <i>forsythia</i> was most susceptible to whole plant and flower extracts (25 μg/ml), followed by leaves extract (100 μg/ml). Finally, <i>A</i>. <i>actinomycetemcomitans</i> was most susceptible to flower extract (25 μg/ml), followed by equal susceptibility at 50 μg/ml concentration of either whole plant or leaves extracts.</p><p><strong>Conclusion: </strong>The <i>S</i>. <i>indicus</i> ethanol extracts of aerial parts, leaves, and flowers showed good antimicrobial activity against periodontal pathogens.</p>","PeriodicalId":15890,"journal":{"name":"Journal of Indian Society of Periodontology","volume":"28 5","pages":"544-550"},"PeriodicalIF":0.0000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932560/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Indian Society of Periodontology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jisp.jisp_397_23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/26 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"Dentistry","Score":null,"Total":0}
引用次数: 0

Abstract

Introduction: Sphaeranthus indicus is a medicinal plant widely used in the Indian traditional system of medicine for curing various ailments. However, there has been no report of any study testing its antimicrobial action against dental or periodontal pathogens. Hence, the aim of this study was to evaluate the in vitro antimicrobial activity of extract of whole plant, leaves, and flowers of S. indicus against four periodontal pathogens, namely, Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans.

Materials and methods: Extracts of the aerial part (whole plant), leaves, and flowers of Sphaeranthus indicus were prepared using cold maceration and ethanol extraction. The extracts were tested for their antimicrobial activity against periodontal pathogens by using in vitro serial dilution method at concentration of 12.5, 25, 50, and 100 μg/ml. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of all three groups were determined against all four periodontal pathogenic using thioglycollate broth and chlorhexidine as a positive control.

Results: P. gingivalis and Fusobacterium nucleatum were most susceptible to leaves extract (12.5 μg/ml and 25 μg/ml) followed by whole aerial plant extract (25 μg/ml and 50 μg/ml), but were least susceptible to flower extract (100 μg/ml). Another pathogen, T. forsythia was most susceptible to whole plant and flower extracts (25 μg/ml), followed by leaves extract (100 μg/ml). Finally, A. actinomycetemcomitans was most susceptible to flower extract (25 μg/ml), followed by equal susceptibility at 50 μg/ml concentration of either whole plant or leaves extracts.

Conclusion: The S. indicus ethanol extracts of aerial parts, leaves, and flowers showed good antimicrobial activity against periodontal pathogens.

Abstract Image

Abstract Image

Abstract Image

球兰地上部分、叶和花提取物对不同牙周病原菌的体外抑菌作用研究。
简介:印度球兰是一种药用植物,在印度传统医学体系中被广泛用于治疗各种疾病。然而,没有任何研究报告测试其对牙齿或牙周病原体的抗菌作用。因此,本研究旨在评价indicus整株提取物、叶片和花提取物对牙龈卟啉单胞菌、连翘单宁菌、核梭菌和放线菌聚集菌4种牙周病原菌的体外抗菌活性。材料与方法:采用冷浸渍法和乙醇提取法分别提取球兰(Sphaeranthus indicus)地上部分(整株)、叶和花的提取物。采用体外连续稀释法,分别在12.5、25、50、100 μg/ml浓度下检测提取物对牙周病原菌的抑菌活性。以巯基乙酸酯肉汤和氯己定为阳性对照,测定3组对4种牙周病原菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果:牙龈假单胞杆菌和核梭杆菌对叶片提取物(12.5 μg/ml和25 μg/ml)最敏感,其次是全地植物提取物(25 μg/ml和50 μg/ml),对花提取物(100 μg/ml)最不敏感。整株和花提取物(25 μg/ml)对连翘最敏感,叶片提取物(100 μg/ml)次之。结果表明,放线菌对花提取物(25 μg/ml)的敏感性最高,对全株提取物和叶片提取物(50 μg/ml)的敏感性次之。结论:梧桐地上部、叶和花乙醇提取物对牙周病原菌具有良好的抑菌活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
1.70
自引率
0.00%
发文量
87
审稿时长
44 weeks
期刊介绍: The Journal of Indian Society of Periodontology publishes original scientific articles to support practice , education and research in the dental specialty of periodontology and oral implantology. Journal of Indian Society of Periodontology (JISP), is the official publication of the Society and is managed and brought out by the Editor of the society. The journal is published Bimonthly with special issues being brought out for specific occasions. The ISP had a bulletin as its publication for a large number of years and was enhanced as a Journal a few years ago
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信