Cobalt-Incorporated Hydroxyapatite Conditioned Media Promotes In Vitro Scratch Wound Healing and Mesenchymal Stem Cell Migration.

IF 5 3区医学 Q1 ENGINEERING, BIOMEDICAL

Journal of Functional Biomaterials Pub Date : 2025-02-20 DOI:10.3390/jfb16030072

Weerapat Leelasangsai, Krongrat Thummachot, Puttita Thammasarnsophon, Autcharaporn Srion, Jintamai Suwanprateeb, Somying Patntirapong

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引用次数: 0

Abstract

Cell migration of mesenchymal stem cells (MSCs) is critical for bone healing and remodeling. Cobalt is a well-known hypoxia mimic, which can enhance MSC migration. Therefore, the objective of this study was to investigate the migratory response of MSCs to a developed cobalt-incorporated hydroxyapatite (HACo) material. HACo was fabricated by a simple ion exchange procedure at concentrations ranging from 40 to 8000 μM into disc shape. HACo discs were incubated in the media and conditioned media (CM; HACo_CM) were collected for MSC culture. HA_CM served as a control. MSCs were cultured until reaching 90% confluence before the wound was generated by scraping. Time-lapse imaging of wound migration was monitored, recorded, and assessed. Statistical analysis was performed by one-way ANOVA followed by a Dunnett's test. The wound area gradually declined from 0 to 40 h for all samples. HACo_CM at 40 µM (HACo40_CM) promoted wound closure at the early period of wound healing. Both HACo40_CM and HACo8000_CM enhanced the distance and velocity of individual cell migration. However, only HACo40_CM affected cell persistence and direction at the early period of cell migration. Exposure to HACo_CM accelerated the speed of MSC migration, which is necessary for wound healing. The migratory ability of individual cells could help the rate of wound healing. Therefore, HACo materials may serve as potential biomaterials for enhanced bone healing.

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来源期刊

Journal of Functional Biomaterials Engineering-Biomedical Engineering

CiteScore

4.60

自引率

4.20%

发文量

226

审稿时长

11 weeks

期刊介绍： Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.