Porous Hydrogels Prepared by Two-Step Gelation Method for Bone Regeneration.

IF 5 3区医学 Q1 ENGINEERING, BIOMEDICAL

Journal of Functional Biomaterials Pub Date : 2025-03-13 DOI:10.3390/jfb16030100

Yongzhi Li, Jiangshan Liu, Jiawei Wei, Li Yuan, Jiaxin Hu, Siluo Dai, Yubao Li, Jidong Li

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引用次数: 0

Abstract

Hierarchical porous hydrogels possess advantageous characteristics that facilitate cell adhesion, promote tissue growth, and enhance angiogenesis and osteogenesis. In this study, porous composite hydrogels were successfully prepared by a two-step gelation method with sodium alginate (SA), gelatin (GEL), and calcium hydrogen phosphate (DCP) as the main components. The fabricated porous hydrogels initially featured small pores (approximately 60 μm), and gradually evolved to large pores (exceeding 250 μm) during the gradual degradation in the cellular microenvironment. In vitro cell culture experiments indicated that these hydrogels could enhance the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells due to the hierarchical porous structure and the incorporation of DCP. Subcutaneous implantation and cranial defect repair experiments in Sprague-Dawley rats further confirmed that the small initial pore size of hydrogel scaffolds can provide more sites for cell adhesion. Additionally, the gradual degradation to form large pores was conducive to cell/tissue growth and blood vessel formation, ultimately being beneficial for vascularized bone regeneration. In summary, this study proposes an innovative strategy for developing porous hydrogels with gradual degradation for functional bone regeneration.

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两步凝胶法制备用于骨再生的多孔水凝胶。

分层多孔水凝胶具有促进细胞粘附，促进组织生长，促进血管生成和成骨的有利特性。本研究以海藻酸钠（SA）、明胶（GEL）和磷酸氢钙（DCP）为主要成分，采用两步凝胶法制备了多孔复合水凝胶。制备的多孔水凝胶最初具有小孔隙（约60 μm），在细胞微环境中逐渐降解，逐渐演变成大孔隙（超过250 μm）。体外细胞培养实验表明，这些水凝胶由于具有分层多孔结构和DCP的掺入，能够促进骨髓间充质干细胞的增殖和成骨分化。Sprague-Dawley大鼠皮下植入及颅骨缺损修复实验进一步证实了水凝胶支架初始孔径小，可以提供更多的细胞粘附位点。此外，逐渐降解形成大孔隙有利于细胞/组织生长和血管形成，最终有利于血管化骨再生。综上所述，本研究提出了一种开发具有逐渐降解功能骨再生的多孔水凝胶的创新策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Functional Biomaterials Engineering-Biomedical Engineering

CiteScore

4.60

自引率

4.20%

发文量

226

审稿时长

11 weeks

期刊介绍： Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.