Oleuropein mitigates acetaminophen overdose-induced kidney injury in male rats by enhancing antioxidant defense and suppressing inflammatory and apoptotic pathways.
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引用次数: 0
Abstract
Acetaminophen (APAP) is a well-known analgesic, and antipyretic drug and its overdose or chronic consumption can lead to kidney damage. Oleuropein (OLE) exhibits various pharmacological properties. This study aimed to determine the possible therapeutic benefits of OLE in improving APAP-induced kidney injury. In this experimental study, 36 male Wistar rats were assigned to six groups (n = 6). The rats initially received a single dose of APAP (500 mg/kg) and then 1 hour later were treated with a single dose of OLE at 50, 100, and 200 mg/kg depending on their groups. 24 hours after treatment with OLE, various indicators including kidney biochemical tests, histopathological changes, oxidative stress markers, and anti-apoptotic and anti-inflammatory parameters were investigated in the renal tissue. OLE (100 mg/kg) significantly decreased serum creatinine, caspase-3, kidney tissue damage score (P < 0.05), malondialdehyde (MDA) (P < 0.01), and increased superoxide dismutase (SOD) and total antioxidant capacity (TAC) (P < 0.05) in the APAP + OLE 100 mg/kg group versus APAP group. Additionally, OLE (200 mg/kg) significantly reduced blood urea nitrogen (BUN), NF-κB, p53, Bax (p < 0.05), serum creatinine, TNF-α, caspase-3, kidney tissue damage score (p < 0.01), MDA, and Bax: Bcl-2 ratio (p < 0.001) in the APAP + OLE 200 mg/kg group versus APAP group. Also, OLE (200 mg/kg) significantly enhanced glutathione peroxidase (GPx), SOD, Bcl-2 (p < 0.05), and TAC (p < 0.01) in the APAP + OLE 200 mg/kg group contrasted to APAP group. However, OLE at 50 mg/kg didn't alter measured parameters. These findings demonstrate that OLE (200 mg/kg) could attenuate acetaminophen-induced kidney injury through its anti-oxidant, anti-inflammatory, and anti-apoptosis properties.
对乙酰氨基酚（APAP）是一种众所周知的镇痛解热药物，过量或长期服用可导致肾脏损害。橄榄苦苷（OLE）具有多种药理特性。本研究旨在确定OLE在改善apap诱导的肾损伤方面可能的治疗益处。本实验将36只雄性Wistar大鼠分为6组（n = 6）。大鼠最初给予单剂量APAP (500 mg/kg)， 1小时后按组分别给予单剂量OLE（50、100、200 mg/kg）。在OLE治疗24小时后，观察肾组织各项指标，包括肾脏生化指标、组织病理学变化、氧化应激标志物、抗凋亡和抗炎参数。OLE （100 mg/kg）显著降低血清肌酐、caspase-3、肾组织损伤评分（P P P P P P P P P P P）
期刊介绍：
Drug and Chemical Toxicology publishes full-length research papers, review articles and short communications that encompass a broad spectrum of toxicological data surrounding risk assessment and harmful exposure. Manuscripts are considered according to their relevance to the journal.
Topics include both descriptive and mechanics research that illustrates the risk assessment implications of exposure to toxic agents. Examples of suitable topics include toxicological studies, which are structural examinations on the effects of dose, metabolism, and statistical or mechanism-based approaches to risk assessment. New findings and methods, along with safety evaluations, are also acceptable. Special issues may be reserved to publish symposium summaries, reviews in toxicology, and overviews of the practical interpretation and application of toxicological data.
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