Characterization of bile microbiota in patients with obstructive jaundice associated with biliary tract diseases.

IF 3 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY
Junxiong Wang, Qi Liu, Shangjia Jin, Boxing Yang, Chong Wang, Yuan Tan, Weimin Feng, Jingjin Tao, He Wang, Yongjun Wang, Shuo Yang, Liyan Cui
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引用次数: 0

Abstract

Background: Cholangiocarcinoma (CHOL), a malignant tumor of the biliary system, is particularly concerning due to its high malignancy and poor prognosis, often leading to obstructive jaundice. The advent of metagenomic sequencing(mNGS) technology has expanded diagnostic capabilities, including the identification of microbes within tumors and their potential role in cancer progression.

Objective: This study aims to explore the bacterial composition in bile from patients with obstructive jaundice of different etiologies and to investigate the association between bile microbiota and biochemical analytes, as well as their potential as biomarkers for diagnosis of obstructive jaundice diseases.

Methods: Bile samples from patients with obstructive jaundice admitted to Beijing Friendship Hospital were collected and subjected to 16S rRNA and metagenomic sequencing. The study included patients diagnosed with benign biliary stricture, gallstone, and cholangiocarcinoma. Clinical data and bile chemical components were analyzed. The potential functional roles of the identified microbiota were predicted using bioinformatics tools.

Results: The study enrolled 13 patients with benign biliary stricture, 19 with gallstones, and 10 with cholangiocarcinoma. Significant differences in bile chemical components and microbial diversity were observed among the groups. The bile microbiota was dominated by distinct phyla and genera across the groups, with Proteobacteria and Fusobacteriota enriched in benign biliary stricture, Firmicutes and Desulfobacterota in cholangiocarcinoma, and Synergistota in gallstone patients. Functional analysis revealed differences in gene functions related to metabolism and other biological processes. A correlation between bile microbiota and biochemical markers was established, and the combination of differential microbiota showed potential as a diagnostic marker for obstructive jaundice of different etiologies.

Conclusion: Bile microbiota varies significantly among patients with obstructive jaundice of different etiologies. The identified microbial signatures and their functional roles could serve as novel diagnostic markers and provide insights into the pathogenesis of biliary diseases.

梗阻性黄疸合并胆道疾病患者的胆汁微生物群特征
背景:胆管癌(cholangocarcinoma, CHOL)是胆道系统的一种恶性肿瘤,因其恶性程度高、预后差,常导致梗阻性黄疸而引起特别关注。宏基因组测序(mNGS)技术的出现扩大了诊断能力,包括鉴定肿瘤内的微生物及其在癌症进展中的潜在作用。目的:本研究旨在探讨不同病因梗阻性黄疸患者胆汁细菌组成,探讨胆汁微生物群与生化分析的关系,以及它们作为梗阻性黄疸疾病诊断的生物标志物的潜力。方法:收集北京友谊医院梗阻性黄疸患者胆汁标本,进行16S rRNA和宏基因组测序。该研究包括诊断为良性胆道狭窄、胆结石和胆管癌的患者。分析临床资料及胆汁化学成分。利用生物信息学工具预测鉴定的微生物群的潜在功能作用。结果:本研究纳入13例良性胆道狭窄患者,19例胆结石患者,10例胆管癌患者。各组胆汁化学成分和微生物多样性差异显著。胆汁微生物群在各组中以不同的门属为主,良性胆道狭窄中富含变形菌门和梭菌门,胆管癌患者中富含厚壁菌门和脱硫菌门,胆结石患者中富含增塑菌门。功能分析揭示了与代谢和其他生物过程相关的基因功能的差异。建立了胆汁微生物群与生化指标之间的相关性,不同微生物群的组合有可能作为不同病因梗阻性黄疸的诊断指标。结论:不同病因梗阻性黄疸患者胆汁菌群差异显著。所鉴定的微生物特征及其功能作用可以作为新的诊断标记,并为了解胆道疾病的发病机制提供新的见解。
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来源期刊
Clinical and Translational Gastroenterology
Clinical and Translational Gastroenterology GASTROENTEROLOGY & HEPATOLOGY-
CiteScore
7.00
自引率
0.00%
发文量
114
审稿时长
16 weeks
期刊介绍: Clinical and Translational Gastroenterology (CTG), published on behalf of the American College of Gastroenterology (ACG), is a peer-reviewed open access online journal dedicated to innovative clinical work in the field of gastroenterology and hepatology. CTG hopes to fulfill an unmet need for clinicians and scientists by welcoming novel cohort studies, early-phase clinical trials, qualitative and quantitative epidemiologic research, hypothesis-generating research, studies of novel mechanisms and methodologies including public health interventions, and integration of approaches across organs and disciplines. CTG also welcomes hypothesis-generating small studies, methods papers, and translational research with clear applications to human physiology or disease. Colon and small bowel Endoscopy and novel diagnostics Esophagus Functional GI disorders Immunology of the GI tract Microbiology of the GI tract Inflammatory bowel disease Pancreas and biliary tract Liver Pathology Pediatrics Preventative medicine Nutrition/obesity Stomach.
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