Henrique Hadad, Laís Kawamata de Jesus, Maísa Pereira-Silva, Maria Eduarda de Freitas Santana Oliveira, Naara Gabriela Monteiro, Pier Paolo Poli, Carlo Maiorana, Roberta Okamoto, Fernando Pozzi Semeghini Guastaldi, Juliano Milanezi de Almeida, Francisley Ávila Souza
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引用次数: 0
Abstract
Objectives: This study aimed to evaluate doxycycline on the prevention of MRONJ.
Materials and methods: Seventy-two Wistar rats were sorted into 3 groups and subjected to tooth extractions. The first group received systemic saline solution (SAL), the second received zoledronic acid (ZA), and the third, besides the systemic ZA, the post-extraction socket received local application of doxycycline (DOXI) gel. Samples were collected and analyzed at 7, 14, and 28 postoperative days.
Results: DOXI group presented higher percentage of vital bone area as compared with ZA at 7 (p = 0.0058), 14 (p < 0.0001), and 28 days (p < 0.0001). TRAP immunolabeling was lower in ZA and DOXI at 7 and 14 days, while OCN immunolabeling was similar between the ZA and DOXI, except at 7 days. At 14 and 28 days, DOXI group presented more collagen type 3 and 1 (p < 0.0001) as compared with ZA. RUNX2, ALP, OCN, and RANKL gene expression was downregulated in ZA at 7 days. Conversely, DOXI upregulated these markers and OPG over time (p < 0.05).
Conclusion: Doxycycline is an effective local agent for preventing MRONJ, favoring alveolar bone healing.
Clinical relevance: Although widely studied, MRONJ remains a significant challenge in dentistry. The search for preventive methods is important to assure safety for in patients under antiresorptive therapy that demand surgical dental treatment. Doxycycline is well-established in dentistry, and the proposal of using this medication as a gel makes it more appealing since it is applied locally and has negligible adverse effects.
期刊介绍：
The journal Clinical Oral Investigations is a multidisciplinary, international forum for publication of research from all fields of oral medicine. The journal publishes original scientific articles and invited reviews which provide up-to-date results of basic and clinical studies in oral and maxillofacial science and medicine. The aim is to clarify the relevance of new results to modern practice, for an international readership. Coverage includes maxillofacial and oral surgery, prosthetics and restorative dentistry, operative dentistry, endodontics, periodontology, orthodontics, dental materials science, clinical trials, epidemiology, pedodontics, oral implant, preventive dentistiry, oral pathology, oral basic sciences and more.
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