A rice DELLA protein OsSLR1 positively regulates rice resistance to southern rice black-streaked dwarf virus infection.

IF 4.3 2区生物学 Q1 PLANT SCIENCES

BMC Plant Biology Pub Date : 2025-03-26 DOI:10.1186/s12870-025-06394-0

Juan Du, Zihang Yang, Lulu Li, Yanjun Li, Hehong Zhang, Jianping Chen, Zongtao Sun

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引用次数: 0

Abstract

Background: In the course of long-term confrontation with pathogens, plants have developed complex defense mechanisms to protect themselves from various pathogens. Previous studies have reported that the gibberellin (GA) signaling pathway negative regulator SLENDER RICE 1 (SLR1) in rice activates jasmonic acid (JA)-mediated broad-spectrum antiviral immunity, but the exploration regarding whether OsSLR1 exerts effects on alternative antiviral immune pathways remains limited.

Results: Here, we identified that OsSLR1 was significantly induced after virus infection and overexpression of OsSLR1 in rice enhance the resistance of rice to southern rice black-streaked dwarf virus (SRBSDV) in rice. Transcriptome analysis revealed that a total of 2,336 differentially expressed genes (DEGs) were detected upon overexpression of OsSLR1 in rice, including 1,607 upregulated genes and 729 downregulated genes. Gene ontology (GO) enrichment analysis and RT-qPCR analysis revealed that genes related to JA and reactive oxygen species (ROS) were significantly upregulated, while genes associated with abscisic acid (ABA) were significantly downregulated.

Conclusions: These results suggest that OsSLR1 positively regulates the antiviral immunity of rice by modulating multiple pathways.

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水稻DELLA蛋白OsSLR1正调控水稻对南方水稻黑条矮缩病毒感染的抗性。

背景：植物在与病原体的长期对抗过程中，形成了复杂的防御机制来保护自己免受各种病原体的侵害。已有研究报道水稻中赤霉素（GA）信号通路负调控因子SLR1激活茉莉酸（JA）介导的广谱抗病毒免疫，但OsSLR1是否对其他抗病毒免疫通路产生影响的探索仍然有限。结果：本研究发现OsSLR1在病毒感染后被显著诱导，水稻中OsSLR1的过表达增强了水稻对南方水稻黑条矮缩病毒（SRBSDV）的抗性。转录组分析显示，水稻OsSLR1过表达共检测到2336个差异表达基因（deg），其中上调基因1607个，下调基因729个。基因本体（GO）富集分析和RT-qPCR分析显示，与JA和活性氧（ROS）相关的基因显著上调，而与脱落酸（ABA）相关的基因显著下调。结论：这些结果表明OsSLR1通过多种途径正向调节水稻抗病毒免疫。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Plant Biology 生物-植物科学

CiteScore

8.40

自引率

3.80%

发文量

539

审稿时长

3.8 months

期刊介绍： BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.