Candidate target genes in sepsis diagnosis and therapy: identifying hub genes with a spotlight on KLRB1.

IF 3.4 3区 医学 Q2 INFECTIOUS DISEASES
Wang Chen, Chen Haoran, Ding Jinqiu, Tang Xinyi, Yu Dian, Xie Yongpeng, Li Xiaomin
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引用次数: 0

Abstract

Background: Sepsis, which causes systemic inflammation and organ failure, is one of the leading causes of death in the intensive care unit (ICU) and an urgent social health problem. However, the pathogenesis and molecular mechanism of sepsis are unclear. Therefore, this study aimed to identify candidate Hub genes during sepsis progression and the candidate target genes for sepsis diagnosis and treatment.

Methods: GSE54514, GSE57065, GSE69528, GSE95233, and GSE131761 datasets were downloaded from public databases, and the differentially expressed genes (DEGs) between healthy and septic patients in each dataset were screened at adjusted P-value < 0.05 and| log2FC| ≥ 0.58. Subsequently, the obtained DEGs in each dataset were intersected to obtain the Hub genes. In addition, the DEGs between patients with better and poor prognoses in datasets GSE54514 and GSE95233 were analyzed after 28 days. The differential expression of Hub genes in septic patients with good and poor prognoses was detected at adjusted P-value < 0.05 and| log2FC| ≥ 0.58. Finally, real-time quantitative polymerase chain reaction (qRT-PCR) was used to verify the bioinformatics results.

Results: In datasets GSE54514, GSE57065, GSE69528, GSE95233 and GSE131761, RNASE2, RNASE3, CTSG, SLPI, TNFAIP6, PGLYRP1 and BLOC1S1 were up-regulated in septic patients, and RPL10A and KLRB1 were down-regulated compared to healthy controls. qRT-PCR confirmed the expression trend of the hub genes except CTSG (which was not differentially expressed). Compared to septic patients with good prognoses, the differential expression of RNASE3 was higher in patients with poor prognoses. Furthermore, qRT-PCR revealed that KLRB1 was the only differentially expressed hub gene with down-regulated expression in sepsis patients with poor prognosis.

Conclusions: The candidate Hub genes closely related to sepsis include KLRB1, RNASE2, RNASE3, CTSG, SLPI, TNFAIP6, PGLYRP1, BLOC1S1, and RPL10A. KLRB1 is the most relevant candidate hub gene among these hub genes in the molecular underpinnings of sepsis, which could be targeted for sepsis detection and treatment.

背景:败血症会导致全身炎症和器官衰竭,是重症监护病房(ICU)的主要死因之一,也是一个紧迫的社会健康问题。然而,脓毒症的发病机制和分子机制尚不清楚。因此,本研究旨在确定脓毒症进展过程中的候选枢纽基因以及脓毒症诊断和治疗的候选靶基因:方法:从公共数据库下载 GSE54514、GSE57065、GSE69528、GSE95233 和 GSE131761 数据集,以调整后的 P 值筛选每个数据集中健康患者和脓毒症患者之间的差异表达基因(DEGs):在 GSE54514、GSE57065、GSE69528、GSE95233 和 GSE131761 数据集中,与健康对照组相比,脓毒症患者的 RNASE2、RNASE3、CTSG、SLPI、TNFAIP6、PGLYRP1 和 BLOC1S1 上调,RPL10A 和 KLRB1 下调。与预后良好的败血症患者相比,预后不良的患者中 RNASE3 的差异表达更高。此外,qRT-PCR显示,KLRB1是唯一一个差异表达的枢纽基因,在预后不良的脓毒症患者中表达下调:结论:与脓毒症密切相关的候选枢纽基因包括 KLRB1、RNASE2、RNASE3、CTSG、SLPI、TNFAIP6、PGLYRP1、BLOC1S1 和 RPL10A。KLRB1 是这些枢纽基因中与脓毒症分子基础最相关的候选枢纽基因,可作为脓毒症检测和治疗的目标基因。
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来源期刊
BMC Infectious Diseases
BMC Infectious Diseases 医学-传染病学
CiteScore
6.50
自引率
0.00%
发文量
860
审稿时长
3.3 months
期刊介绍: BMC Infectious Diseases is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of infectious and sexually transmitted diseases in humans, as well as related molecular genetics, pathophysiology, and epidemiology.
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