Comparative Release of Platelet-Derived Growth Factor-AA and Evaluation of Osteoblastic Proliferation of Two Liquid Platelet-Rich Fibrin Formulations (C-PRF and I-PRF): An In Vitro Study.
Nithyakalyani Ramesh, Jayanthi Anbalagan, Muthukumar Santhanakrishnan, Alan M Punnoose, Rajalakshmi Shanmugham, John Kirubaharan
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引用次数: 0
Abstract
Context: Several biomaterials have been developed in the field of tissue regeneration, in addition to creating a "foreign body reaction," they lack the cellular components that are necessary for the regeneration process and, therefore, do not fulfill their purpose satisfactorily. In this regard, the use of platelet concentrates has gained popularity. However, sufficient scientific evidence is still lacking for the use of platelet concentrates, especially platelet-rich liquid fibrin preparations in combination with xenografts. The results of the present study would give an indication of the advantages of using the combination of xenogenic bone graft in combination with liquid formulations of platelet concentrates in regenerative periodontal therapy. Aims: This in vitro study was performed to compare and evaluate the differential release profile and osteogenic potential of liquid formulations of platelet concentrates, namely, concentrated platelet-rich fibrin (C-PRF) and injectable platelet-rich fibrin (I-PRF). Methods and Materials: The differential release profile of platelet-derived growth factor-AA (PDGF-AA) and osteogenic potential of liquid formulations of platelet concentrates was evaluated using samples collected from four periodontally healthy female volunteers by ELISA and alkaline phosphatase (ALP) assay with the help of human osteosarcoma cell lines (Saos-2). Statistical Analysis: Statistical analysis of growth factor release profile and estimation of ALP activity was performed using the Kruskal-Wallis test to compare the mean difference between the following groups: C-PRF and I-PRF with and without bone graft. Data were analyzed using SPSS Version 21 software. Results and Conclusions: This study clearly shows the advantage of using liquid platelet concentrates in combination with bone grafts compared with bone grafts alone. The study further suggested that the use of C-PRF could be beneficial in regenerative periodontal therapy.
背景:在组织再生领域已经开发了几种生物材料,除了产生“异物反应”外,它们缺乏再生过程所必需的细胞成分,因此不能令人满意地实现其目的。在这方面,使用血小板浓缩物已经得到普及。然而,对于血小板浓缩物,特别是富含血小板的液体纤维蛋白制剂与异种移植联合使用,仍然缺乏足够的科学证据。本研究结果提示异种骨移植联合血小板浓缩液在再生牙周治疗中的优势。目的:本体外研究比较和评价血小板浓缩液(即浓缩富血小板纤维蛋白(C-PRF)和可注射富血小板纤维蛋白(I-PRF))的差异释放谱和成骨潜能。方法与材料:采用ELISA法和碱性磷酸酶(ALP)测定法,利用人骨肉瘤细胞系(Saos-2)检测4例牙周健康女性受试者的血小板衍生生长因子- aa (PDGF-AA)的差异释放谱和成骨潜能。统计学分析:采用Kruskal-Wallis检验对生长因子释放谱和ALP活性进行统计分析,比较C-PRF组和I-PRF组与未植骨组之间的平均差异。数据分析采用SPSS Version 21软件。结果与结论:本研究清楚地显示了液体血小板浓缩物联合骨移植相比单独骨移植的优势。该研究进一步表明,使用C-PRF可能有利于再生牙周治疗。