Exploiting the 5-amino-11H-indolo[3,2-c]isoquinoline Core to Achieve Better G-quadruplex Ligands for Cancer Therapy.

IF 3.6 4区 医学 Q2 CHEMISTRY, MEDICINAL
ChemMedChem Pub Date : 2025-03-26 DOI:10.1002/cmdc.202401019
Israa M Aljnadi, Barbara Bahls, Noélia Duarte, Bruno L Victor, Eduarda Mendes, Sergio P Camões, Joana P Miranda, Ermelinda Maçôas, Alexandra Paulo
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引用次数: 0

Abstract

G-quadruplexes (G4) are secondary structures that can form within guanine-rich  nucleic acids and have cell proliferation regulatory functions. Targeting DNA G4 structures has emerged as a promising anticancer therapy, highlighting the need for new G4 ligands with reduced number of cationic groups to ensure lower toxicity. In this study, we report the synthesis of mono- and di-substituted 5-amino-11H-indolo[3,2-c]isoquinolines. Fluorescence spectroscopy studies indicate that substitution in position 11 dictates the preference of binding to different G4. Compound 10, which features a ethylpyrrolidine side chain, demonstrated a binding preference by one order of magnitude for parallel c-MYCG4 (Kb = 107 M-1), over parallel k-RASG4 (Kb = 106 M-1), hybrid TeloG4 and dsDNA (Kb = 105 M-1). Molecular docking studies revealed that compound 10 can bind not only to the flat G-quartets but also to bridge between two loops of c-MYCG4 through hydrogen bonds, which may explain its capacity to discriminate between G4. Moreover, compound 10 drastically reduced the cell viability of breast cancer cells at a concentration of 10 µM. Overall, herein we report the discovery of a new potent and selective G4 ligand, with reduced number of side chains and antiproliferative activity in cancer cells that deserves to be further investigated.

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来源期刊
ChemMedChem
ChemMedChem 医学-药学
CiteScore
6.70
自引率
2.90%
发文量
280
审稿时长
1 months
期刊介绍: Quality research. Outstanding publications. With an impact factor of 3.124 (2019), ChemMedChem is a top journal for research at the interface of chemistry, biology and medicine. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies. ChemMedChem publishes primary as well as critical secondary and tertiary information from authors across and for the world. Its mission is to integrate the wide and flourishing field of medicinal and pharmaceutical sciences, ranging from drug design and discovery to drug development and delivery, from molecular modeling to combinatorial chemistry, from target validation to lead generation and ADMET studies. ChemMedChem typically covers topics on small molecules, therapeutic macromolecules, peptides, peptidomimetics, and aptamers, protein-drug conjugates, nucleic acid therapies, and beginning 2017, nanomedicine, particularly 1) targeted nanodelivery, 2) theranostic nanoparticles, and 3) nanodrugs. Contents ChemMedChem publishes an attractive mixture of: Full Papers and Communications Reviews and Minireviews Patent Reviews Highlights and Concepts Book and Multimedia Reviews.
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