Exploiting the 5-amino-11H-indolo[3,2-c]isoquinoline Core to Achieve Better G-quadruplex Ligands for Cancer Therapy.

IF 3.6 4区 医学 Q2 CHEMISTRY, MEDICINAL
ChemMedChem Pub Date : 2025-03-26 DOI:10.1002/cmdc.202401019
Israa M Aljnadi, Barbara Bahls, Noélia Duarte, Bruno L Victor, Eduarda Mendes, Sergio P Camões, Joana P Miranda, Ermelinda Maçôas, Alexandra Paulo
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引用次数: 0

Abstract

G-quadruplexes (G4) are secondary structures that can form within guanine-rich  nucleic acids and have cell proliferation regulatory functions. Targeting DNA G4 structures has emerged as a promising anticancer therapy, highlighting the need for new G4 ligands with reduced number of cationic groups to ensure lower toxicity. In this study, we report the synthesis of mono- and di-substituted 5-amino-11H-indolo[3,2-c]isoquinolines. Fluorescence spectroscopy studies indicate that substitution in position 11 dictates the preference of binding to different G4. Compound 10, which features a ethylpyrrolidine side chain, demonstrated a binding preference by one order of magnitude for parallel c-MYCG4 (Kb = 107 M-1), over parallel k-RASG4 (Kb = 106 M-1), hybrid TeloG4 and dsDNA (Kb = 105 M-1). Molecular docking studies revealed that compound 10 can bind not only to the flat G-quartets but also to bridge between two loops of c-MYCG4 through hydrogen bonds, which may explain its capacity to discriminate between G4. Moreover, compound 10 drastically reduced the cell viability of breast cancer cells at a concentration of 10 µM. Overall, herein we report the discovery of a new potent and selective G4 ligand, with reduced number of side chains and antiproliferative activity in cancer cells that deserves to be further investigated.

利用5-氨基- 11h -吲哚[3,2-c]异喹啉核心获得用于癌症治疗的更好的g -四联体配体。
g -四plex (G4)是富鸟嘌呤核酸内可形成的二级结构,具有细胞增殖调节功能。靶向DNA G4结构已成为一种很有前景的抗癌疗法,强调需要新的G4配体,减少阳离子基团的数量,以确保降低毒性。在这项研究中,我们报道了单和双取代5-氨基- 11h -吲哚[3,2-c]异喹啉的合成。荧光光谱研究表明,11位的取代决定了与不同G4的结合偏好。化合物10具有一个乙基吡啶侧链,与平行的c-MYCG4 (Kb = 107 M-1)、k-RASG4 (Kb = 106 M-1)、杂交的TeloG4和dsDNA (Kb = 105 M-1)相比,前者的结合偏好高一个数量级。分子对接研究表明,化合物10不仅可以与平面g -四边形结合,还可以通过氢键连接c-MYCG4的两个环,这可能解释了其区分G4的能力。此外,化合物10在浓度为10µM时可显著降低乳腺癌细胞的活力。总之,本文报告了一种新的强效和选择性G4配体的发现,其侧链数量减少,在癌细胞中具有抗增殖活性,值得进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ChemMedChem
ChemMedChem 医学-药学
CiteScore
6.70
自引率
2.90%
发文量
280
审稿时长
1 months
期刊介绍: Quality research. Outstanding publications. With an impact factor of 3.124 (2019), ChemMedChem is a top journal for research at the interface of chemistry, biology and medicine. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies. ChemMedChem publishes primary as well as critical secondary and tertiary information from authors across and for the world. Its mission is to integrate the wide and flourishing field of medicinal and pharmaceutical sciences, ranging from drug design and discovery to drug development and delivery, from molecular modeling to combinatorial chemistry, from target validation to lead generation and ADMET studies. ChemMedChem typically covers topics on small molecules, therapeutic macromolecules, peptides, peptidomimetics, and aptamers, protein-drug conjugates, nucleic acid therapies, and beginning 2017, nanomedicine, particularly 1) targeted nanodelivery, 2) theranostic nanoparticles, and 3) nanodrugs. Contents ChemMedChem publishes an attractive mixture of: Full Papers and Communications Reviews and Minireviews Patent Reviews Highlights and Concepts Book and Multimedia Reviews.
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