Fecal Secretory Immunoglobulin A and Lactate Level as a Biomarker of Mucosal Immune Dysfunction in Horses With Colic

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine Pub Date : 2025-03-27 DOI:10.1111/jvim.70073

Agnieszka Żak-Bochenek, Zuzana Drábková, Vaiva Sergedaite, Natalia Siwińska, Joanna Bajzert, Dominika Pasak, Anna Chełmońska-Soyta

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Abstract

Background

Colic-related obstructions can reduced intestinal mucosa function and cause dysbiosis in horses, but it is unclear how defense barrier and secretory immunoglobulin A (SIgA) secretion is disrupted.

Objectives

The aim of the study is to evaluate the effect of severity of colic signs and treatments on fecal SIgA and fecal lactate in horses.

Animals

Sixty-two client owned hospitalised horses with colic and eight healthy horses.

Methods

Prospective clinical trial. Fecal samples were taken daily for 7 days. SIgA was analyzed using ELISA, and D/L-lactate measured with a commercial kit.

Results

At Day 0, SIgA values in the colic medical and colic surgical groups were significantly higher than in the control stable group (U = 126.0, p = 0.099, Cliff's ∆ = 0.58 and U = 248.0, p = 0.005, Cliff's ∆ = 0.72, respectively). We found significant correlation between fecal SIgA and fecal lactate level in D0 (r_s = 0.421, p = 0.038).

Conclusions

This study demonstrates the feasibility of using fecal samples to identify biomarkers of colic in horses. An increase in fecal SIgA in horses with colic might suggest the presence of inflammation within the intestines and disruption of the mucosal barrier. These data highlight changes in gastrointestinal barrier and immune function and the intestinal microbiota's metabolic activity in horses with colic.

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马肠绞痛患者粪便分泌免疫球蛋白A和乳酸水平作为黏膜免疫功能障碍的生物标志物

肠绞痛相关的梗阻可降低马的肠黏膜功能并导致生态失调，但防御屏障和分泌免疫球蛋白A （SIgA）的分泌是如何被破坏的尚不清楚。目的探讨不同程度的绞痛症状和治疗方法对马粪便SIgA和乳酸的影响。62个客户有住院的马绞痛和8个健康的马。方法前瞻性临床试验。每天采集粪便样本，连续7天。ELISA法测定SIgA，商用试剂盒测定D/ l -乳酸。结果第0天，绞痛内科组和绞痛外科组SIgA值显著高于对照稳定组（U = 126.0, p = 0.099, Cliff’s∆= 0.58;U = 248.0, p = 0.005, Cliff’s∆= 0.72）。我们发现粪便SIgA与D0粪乳酸水平显著相关（rs = 0.421, p = 0.038）。结论本研究证明了利用粪便样本识别马肠绞痛生物标志物的可行性。马肠绞痛患者粪便中SIgA的增加可能表明肠道内存在炎症和粘膜屏障的破坏。这些数据强调了肠绞痛马的胃肠道屏障和免疫功能以及肠道微生物群代谢活性的变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Internal Medicine 农林科学-兽医学

CiteScore

4.50

自引率

11.50%

发文量

243

审稿时长

22 weeks

期刊介绍： The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.