Rapid point-of-need blood fluke detection in Southern Bluefin Tuna samples using recombinase polymerase amplification coupled with lateral flow test (RPA-LF)

IF 2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2025-03-22 DOI:10.1016/j.vetpar.2025.110457

Cecilia Power , Melissa J. Carabott , Luke Norbury , Kirsten Rough , Barbara F. Nowak , Nathan J. Bott

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引用次数: 0

Abstract

Aporocotylid blood flukes Cardicola forsteri and C. orientalis are considered one of the most significant health concerns for Southern Bluefin Tuna (SBT) Thunnus maccoyii ranched in Australia. There is a need for rapid point-of-need diagnostics to detect Cardicola spp. in SBT to allow the industry to make timely management decisions. Recombinase polymerase amplification (RPA) is an isothermal technique which operates at constant low temperature (25–42˚C), and when coupled with a lateral flow (LF) strip, makes an ideal diagnostic tool for rapid, specific, and sensitive identification of pathogens in field applications. RPA-LF assays were designed and validated for detection of C. forsteri and C. orientalis. For each assay, no cross-species amplification was seen and detection as low as 30–50 gene copy equivalents was achieved. Reactions can be completed in 10 minutes. Similar specificity and sensitivity were demonstrated for SBT samples when compared to qPCR analysis, and use of equipment-free incubation using body heat outside of laboratory settings was demonstrated. By developing rapid, ready-to-use diagnostics, the SBT industry can identify risks relating to blood flukes far quicker than is currently possible.

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来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.