An assessment of sulfide intrusion, genotypic diversity, and clone size in the seagrass Halodule wrightii

IF 1.9 4区生物学 Q2 MARINE & FRESHWATER BIOLOGY

Aquatic Botany Pub Date : 2025-03-21 DOI:10.1016/j.aquabot.2025.103882

Allyson Girard, Sebastian Rubiano-Rincon, Amanda Burton, Patrick D. Larkin

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Abstract

Genetic, including genotypic, diversity is positively associated with traits important for the maintenance of seagrass populations, while clonal reproduction has been shown to be correlated with an increased probability of survival, especially in stressful environments. We performed a pilot study to investigate the relationship between the intrusion of hydrogen sulfide (“sulfide”), an environmental phytotoxin, with genotypic diversity and clone size in the seagrass Halodule wrightii. Isotope ratio mass spectrometry (IRMS) was used to collect sulfur isotopic (δ³⁴S) data to measure sulfide intrusion in 143 root, rhizome, and leaf tissue samples from three sites in the western Gulf of Mexico. A series of microsatellite markers and gridded coordinates were used for genotyping, clonal type (single- vs multi-ramet) and size estimation. While individual genotypes varied for sulfide intrusion, multi-way ANOVA identified location to be a more important factor. Environmental attributes appear to have a more significant role than genotypic identity, clonal type, or size for sulfide uptake and distribution in this species.

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海草中硫化物入侵、基因型多样性和克隆大小的评价

遗传（包括基因型）多样性与维持海草种群的重要特征呈正相关，而克隆繁殖已被证明与生存概率增加相关，特别是在压力环境中。本文对环境毒素硫化氢（“硫化氢”）入侵与海草基因型多样性和克隆大小的关系进行了初步研究。采用同位素比质谱法（IRMS）收集硫同位素（δ34S）数据，测定了墨西哥湾西部3个地点143个根、根茎和叶组织样品的硫化物入侵情况。利用一系列微卫星标记和网格坐标进行基因分型、克隆型（单系与多系）和大小估计。虽然硫化物入侵的个体基因型不同，但多因素方差分析发现位置是更重要的因素。环境属性似乎比基因型、克隆类型或大小对硫化物的吸收和分布有更重要的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquatic Botany 生物-海洋与淡水生物学

CiteScore

3.80

自引率

5.60%

发文量

审稿时长

6 months

期刊介绍： Aquatic Botany offers a platform for papers relevant to a broad international readership on fundamental and applied aspects of marine and freshwater macroscopic plants in a context of ecology or environmental biology. This includes molecular, biochemical and physiological aspects of macroscopic aquatic plants as well as the classification, structure, function, dynamics and ecological interactions in plant-dominated aquatic communities and ecosystems. It is an outlet for papers dealing with research on the consequences of disturbance and stressors (e.g. environmental fluctuations and climate change, pollution, grazing and pathogens), use and management of aquatic plants (plant production and decomposition, commercial harvest, plant control) and the conservation of aquatic plant communities (breeding, transplantation and restoration). Specialized publications on certain rare taxa or papers on aquatic macroscopic plants from under-represented regions in the world can also find their place, subject to editor evaluation. Studies on fungi or microalgae will remain outside the scope of Aquatic Botany.