Probiotic Lactiplantibacillus plantarum Lb41 alleviates high-fat diet-induced nonalcoholic fatty liver disease in mice

IF 3.2 3区医学 Q2 NUTRITION & DIETETICS

Nutrition Pub Date : 2025-02-28 DOI:10.1016/j.nut.2025.112735

Na-Kyoung Lee , Yunjung Lee , Da-Soul Shin , Eunju Park , Hyun-Dong Paik

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引用次数: 0

Abstract

Objective

Nonalcoholic fatty liver disease (NAFLD) is mainly related to genetics, obesity, insulin resistance, and type 2 diabetes. Probiotic Lactiplantibacillus plantarum Lb41 (Lb41) has not been reported to have hepatoprotective effects. Therefore, the aim of this study is to investigate the preventive effects of Lb41 against NAFLD in high-fat diet (HFD)-fed mice for preventing NAFLD.

Method

To induce fatty liver, the mice were given HFD for 5 weeks, followed by silymarin (200 mg/kg) or Lb41 (10⁸ or 10⁹ colony forming units/day) with the HFD for 7 weeks. After 12 weeks, body weight, histological change, serum and hepatic lipid profiles, etc. was performed compared to control and silymarin.

Results

Lb41 had significantly reduced body weight (4.87 g) and serum lipids (triglycerides (77.64%), total cholesterol (67.53%), and low-density lipoprotein (40.50%) compared with the HFD group (P < 0.05). Lb41 significantly relieved HFD-associated hepatic injury by reducing aspartate transaminase (0.49–0.57 fold), alanine transaminase (0.49–0.51 fold), and alkaline phosphatase (0.76–0.90 fold) (P < 0.05). Additionally, they had decreased expression levels of peroxisome proliferator-activated receptor (PPAR) γ and sterol regulatory element-binding protein 1c and increased the expression levels of acyl-CoA oxidase, PPARα, carnitine palmitoyltransferase 1, acetyl CoA carboxylase 1, and fatty acid synthase in liver cells. Insulin and leptin levels decreased in the Lb41 treatment group compared with those in the HFD group. Meanwhile, adiponectin levels increased, similar to those in the normal diet group.

Conclusion

Based on these findings, Lb41 probiotics have possible hepatoprotective effects and could be used as functional food materials.

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益生菌植物乳杆菌Lb41减轻小鼠高脂肪饮食诱导的非酒精性脂肪肝疾病

目的非酒精性脂肪性肝病（NAFLD）主要与遗传、肥胖、胰岛素抵抗和2型糖尿病相关。益生菌植物乳酸杆菌Lb41 （Lb41）没有肝保护作用的报道。因此，本研究旨在探讨Lb41对高脂饮食（HFD）喂养小鼠NAFLD的预防作用。方法用HFD诱导小鼠脂肪肝5周，再用水飞蓟素（200 mg/kg）或Lb41（108、109菌落形成单位/天）灌胃7周。12周后，与对照组和水飞蓟素组进行体重、组织学变化、血清和肝脏脂质等比较。结果与HFD组相比，slb41组患者体重（4.87 g）、血脂(甘油三酯（77.64%）、总胆固醇（67.53%）、低密度脂蛋白（40.50%）均显著降低(P <；0.05)。Lb41通过降低天冬氨酸转氨酶（0.49-0.57倍）、丙氨酸转氨酶（0.49-0.51倍）和碱性磷酸酶（0.76-0.90倍）显著缓解手足口病相关肝损伤(P <；0.05)。此外，肝细胞中过氧化物酶体增殖物激活受体(PPAR) γ和甾醇调节元件结合蛋白1c的表达水平降低，酰基辅酶a氧化酶、PPARα、肉碱棕榈酰基转移酶1、乙酰辅酶a羧化酶1和脂肪酸合成酶的表达水平升高。与HFD组相比，Lb41治疗组的胰岛素和瘦素水平下降。与此同时，脂联素水平上升，与正常饮食组相似。结论Lb41益生菌具有一定的保肝作用，可作为功能性食品原料。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nutrition 医学-营养学

CiteScore

7.80

自引率

2.30%

发文量

300

审稿时长

60 days

期刊介绍： Nutrition has an open access mirror journal Nutrition: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Founded by Michael M. Meguid in the early 1980''s, Nutrition presents advances in nutrition research and science, informs its readers on new and advancing technologies and data in clinical nutrition practice, encourages the application of outcomes research and meta-analyses to problems in patient-related nutrition; and seeks to help clarify and set the research, policy and practice agenda for nutrition science to enhance human well-being in the years ahead.