Thymol rich essential oil from Assam ecotype of Ocimum gratissimum as a biocontrol agent for sheath blight disease of rice

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2025-03-20 DOI:10.1016/j.bcab.2025.103563

Alinaj Yasin , Bhaskar Dowarah , Rosie Brahma , Rijubanki Challam , Chayanika Bora , Pragya Permita Acharyya , Olympica Das , Amit Kumar Das , Manoj Kumar Deka , Anurag Kashyap

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引用次数: 0

Abstract

Preventing crop losses caused by harmful plant pathogens through eco-friendly ways is paramount for food security and environmental sustainability. Envisioning this, the current study evaluates the biocontrol potential of essential oil extracted from Ocimum gratissimum from an Assam ecotype and its primary component, thymol, against Rhizoctonia solani, the pathogen causing sheath blight disease in rice. GC-MS analysis identified thymol as the dominant compound in the essential oil, contributing significantly to its antifungal activity. Both O. gratissimum essential oil and thymol showed strong in-vitro inhibition of R. solani, with complete growth inhibition at 1000 ppm, while thymol exhibited superior efficacy. Both agents also effectively inactivated sclerotia, a key survival mechanism of the pathogen. In-planta assays demonstrated a significant reduction in lesion size with O. gratissimum essential oil and thymol treatments, outperforming the chemical fungicide propiconazole. Thymol was particularly effective in reducing disease severity, making it a promising eco-friendly alternative. The unique phytochemical profile of the Assam ecotype of O. gratissimum further underscores its potential for sustainable disease management. These results underscore the potential of O. gratissimum essential oil and thymol as eco-friendly alternatives to synthetic fungicides. By reducing reliance on chemical inputs, their application can mitigate risks associated with fungicide resistance and environmental contamination while promoting sustainable disease management strategies.

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来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.