Proteomic analysis reveals a potential anticancer mechanism for a novel effervescent curcumin-ascorbic acid-polysaccharide-β-cyclodextrin inclusion complex towards colorectal cancer cells through modulation of ribosome biogenesis

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications Pub Date : 2025-03-22 DOI:10.1016/j.carpta.2025.100775

Atthapan Morchang , Keerakarn Somsuan , Artitaya Rongjumnong , Churat Weeraphan , Sasivimon Pramual , Theetat Ruangjaroon , Daranee Chokchaichamnankit , Wuttichai Jaidee , Phateep Hankittichai , Chanatip Pramvichai , Rawiwan Charoensup , Arunothai Wanta , Chantragan Srisomsap , Jisnuson Svasti , Siripat Aluksanasuwan

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Abstract

Colorectal cancer (CRC) is a major global health issue due to its aggressiveness and high mortality rates. While curcumin, the bioactive compound from turmeric (Curcuma longa L.), exhibits anticancer properties, its low water solubility limits therapeutic potential. The present study developed a novel effervescent curcumin-ascorbic acid-polysaccharide-β-cyclodextrin (CUR-A-Poly-β-CD) inclusion complex to enhance solubility and evaluated its anticancer potential in CRC cell lines. The complex exhibited a zeta potential of -22.9 ± 1.1 mV, a particle size of 496.8 ± 1.9 nm, a solubility of 13.6 mg/L, and a pH of 4.5. CUR-A-Poly-β-CD was non-toxic to normal colon cells (CCD-841 CoN) but significantly reduced the viability of CRC cell lines HT-29, SW-48, and SW-480, with SW-480 cells being the most responsive. CUR-A-Poly-β-CD induced apoptosis and inhibited proliferation, clonogenicity, and migration of SW-480 cells. Proteomic analysis identified 105 altered proteins in SW-480 cells treated with CUR-A-Poly-β-CD, primarily involved in cell growth and ribosome biogenesis pathways. Western blot analysis confirmed increased levels of ribosomal proteins RPL13a and RPS3. In conclusion, the CUR-A-Poly-β-CD inclusion complex demonstrates anticancer effects against CRC by modulating ribosome biogenesis. These findings suggest its potential as a therapeutic approach to improve the effectiveness of CRC treatments.

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大肠癌（CRC）因其侵袭性和高死亡率而成为全球主要的健康问题。姜黄素是姜黄（Curcuma longa L.）中的生物活性化合物，具有抗癌特性，但其水溶性较低，限制了治疗潜力。本研究开发了一种新型泡腾姜黄素-抗坏血酸-多糖-β-环糊精（CUR-A-Poly-β-CD）包合复合物，以提高其溶解度，并评估了其在 CRC 细胞系中的抗癌潜力。该复合物的 zeta 电位为 -22.9 ± 1.1 mV，粒径为 496.8 ± 1.9 nm，溶解度为 13.6 mg/L，pH 值为 4.5。CUR-A-Poly-β-CD 对正常结肠细胞（CCD-841 CoN）无毒性，但会显著降低 CRC 细胞系 HT-29、SW-48 和 SW-480 的存活率，其中 SW-480 细胞的反应性最强。CUR-A-Poly-β-CD 可诱导 SW-480 细胞凋亡并抑制其增殖、克隆性和迁移。蛋白质组分析发现，经 CUR-A-Poly-β-CD 处理的 SW-480 细胞中有 105 种蛋白质发生了改变，主要涉及细胞生长和核糖体生物生成途径。Western 印迹分析证实了核糖体蛋白 RPL13a 和 RPS3 水平的升高。总之，CUR-A-Poly-β-CD 包合物通过调节核糖体的生物生成，对 CRC 具有抗癌作用。这些研究结果表明，CUR-A-聚-β-CD包合物有可能成为一种提高 CRC 治疗效果的治疗方法。

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来源期刊

Carbohydrate Polymer Technologies and Applications

CiteScore

8.70

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0.00%

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