Nanoparticles Pt-Pd (PAPLAL) catalyze tetrahydrobiopterin oxidation and this contributes to the therapy and phototherapy of vitiligo

IF 4.1 3区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Photochemistry and Photobiology A-chemistry Pub Date : 2025-03-23 DOI:10.1016/j.jphotochem.2025.116404

T.A. Telegina , Yu.L. Vechtomova , D.A. Makarova , A.S. Nizamutdinov , A.A. Buglak

{"title":"Nanoparticles Pt-Pd (PAPLAL) catalyze tetrahydrobiopterin oxidation and this contributes to the therapy and phototherapy of vitiligo","authors":"T.A. Telegina , Yu.L. Vechtomova , D.A. Makarova , A.S. Nizamutdinov , A.A. Buglak","doi":"10.1016/j.jphotochem.2025.116404","DOIUrl":null,"url":null,"abstract":"<div><div>Vitiligo is a dermatological autoimmune disease characterized by the loss of melanin pigment. The pathogenesis of vitiligo involves oxidative stress and the autoimmune response mediated by it. Given the current state of vitiligo treatment and the role of tetrahydrobiopterin (H<sub>4</sub>Bip) as a trigger of vitiligo, the purpose of this work is to study the effect of Pt-Pd nanoparticles (NPs) on the auto- and photooxidation H<sub>4</sub>Bip as a therapy for vitiligo.</div><div>Electron microscopy determined: the diameter of palladium NPs was 3.59 ± 0.56 nm and the diameter of platinum NPs was 1.93 ± 0.34 nm. The zeta potential was −16.9 ± 3.4 mV. It was demonstrated that Pt-Pd NPs catalyze the autoxidation of H<sub>4</sub>Bip to dihydropterins and their catalytic activity is proportional to the Pt-Pd NPs concentration. The therapeutic effect of NPs may be due to the fact that dihydropterins do not inhibit melanogenesis. NPs also catalyze the formation of dihydropterin dimers upon irradiation of H<sub>4</sub>Bip solutions at 308 and 325 nm. The removal of H<sub>4</sub>Bip by photooxidation is apparently the basis for phototherapy of vitiligo.</div><div>We have shown for the first time that, along with catalase-like and superoxide dismutase-like effects, one of the main pathways of action of Pt-Pd NPs is the catalysis of the oxidation of H<sub>4</sub>Bip to dihydropterins and their conversion into dimers under UV irradiation <em>in situ</em>. Thus, Pt-Pd NPs are apparently able to remove excess of H<sub>4</sub>Bip and thereby prevent the development of strong oxidative stress leading to the development of vitiligo.</div></div>","PeriodicalId":16782,"journal":{"name":"Journal of Photochemistry and Photobiology A-chemistry","volume":"466 ","pages":"Article 116404"},"PeriodicalIF":4.1000,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Photochemistry and Photobiology A-chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1010603025001443","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Vitiligo is a dermatological autoimmune disease characterized by the loss of melanin pigment. The pathogenesis of vitiligo involves oxidative stress and the autoimmune response mediated by it. Given the current state of vitiligo treatment and the role of tetrahydrobiopterin (H₄Bip) as a trigger of vitiligo, the purpose of this work is to study the effect of Pt-Pd nanoparticles (NPs) on the auto- and photooxidation H₄Bip as a therapy for vitiligo.

Electron microscopy determined: the diameter of palladium NPs was 3.59 ± 0.56 nm and the diameter of platinum NPs was 1.93 ± 0.34 nm. The zeta potential was −16.9 ± 3.4 mV. It was demonstrated that Pt-Pd NPs catalyze the autoxidation of H₄Bip to dihydropterins and their catalytic activity is proportional to the Pt-Pd NPs concentration. The therapeutic effect of NPs may be due to the fact that dihydropterins do not inhibit melanogenesis. NPs also catalyze the formation of dihydropterin dimers upon irradiation of H₄Bip solutions at 308 and 325 nm. The removal of H₄Bip by photooxidation is apparently the basis for phototherapy of vitiligo.

We have shown for the first time that, along with catalase-like and superoxide dismutase-like effects, one of the main pathways of action of Pt-Pd NPs is the catalysis of the oxidation of H₄Bip to dihydropterins and their conversion into dimers under UV irradiation in situ. Thus, Pt-Pd NPs are apparently able to remove excess of H₄Bip and thereby prevent the development of strong oxidative stress leading to the development of vitiligo.

Abstract Image

查看原文本刊更多论文

纳米Pt-Pd （PAPLAL）催化四氢生物蝶呤氧化，有助于白癜风的治疗和光疗

白癜风是一种以黑色素丧失为特征的皮肤自身免疫性疾病。白癜风的发病机制涉及氧化应激及其介导的自身免疫反应。鉴于目前白癜风的治疗现状，以及四氢生物蝶呤（H4Bip）作为白癜风的触发因子的作用，本研究的目的是研究Pt-Pd纳米颗粒（NPs）对H4Bip的自氧化和光氧化作用作为白癜风的治疗方法。电镜测定：钯NPs直径为3.59±0.56 nm，铂NPs直径为1.93±0.34 nm。zeta电位为−16.9±3.4 mV。结果表明，Pt-Pd NPs催化H4Bip自氧化生成双氢蝶呤，其催化活性与Pt-Pd NPs浓度成正比。NPs的治疗效果可能是由于双氢蝶呤不抑制黑色素生成。在H4Bip溶液308和325 nm照射下，NPs还催化形成二氢蝶呤二聚体。通过光氧化去除H4Bip显然是白癜风光疗的基础。除了过氧化氢酶和超氧化物歧化酶的作用外，我们首次发现Pt-Pd NPs的主要作用途径之一是在紫外线照射下催化H4Bip氧化为二氢蝶呤并将其转化为二聚体。因此，Pt-Pd NPs显然能够去除过量的H4Bip，从而防止导致白癜风发展的强氧化应激的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Photochemistry and Photobiology A-chemistry 化学-物理化学

CiteScore

7.90

自引率

7.00%

发文量

580

审稿时长

48 days

期刊介绍： JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds. All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor). The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.