Structure, rheology and antioxidant properties of a polysaccharide from Atractylodes macrocephala in Pan'an

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2025-03-21 DOI:10.1016/j.carres.2025.109464

Min Lin , Guodong Wang , Yunzhi Li

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Abstract

To date, no studies have reported on the structure and properties of the polysaccharides from Atractylodes macrocephala cultivated in Pan'an. In this paper, a polysaccharide designated as RAMP1 was isolated from A. macrocephala in Pan'an for the first time. Its structure was determined as a heteropolysaccharide consisting of fructose and glucose in a molar ratio of 20:1, with a molecular weight of 3494 Da based on chemical and NMR analysis. Scanning electron microscopy analysis revealed that RAMP1 presented a smooth flake structure with irregular curls. Thermal analysis results indicated that RAMP1 had high thermal stability below 165 °C. Rheological studies indicated that both temperature and concentration affected the viscosity of RAMP1. The viscosity of the RAMP1 solution decreased as the shear rate increased, displaying shear - thinning behavior, suggesting that the RAMP1 solution was a non - Newtonian fluid. RAMP1 showed potent dose - dependent antioxidant activity against DPPH, superoxide, ABTS, and hydroxyl radicals, with the strongest inhibition against hydroxyl radicals (83.97 ± 1.62 % at 8 mg/mL). This paper reports for the first time on the structure, rheology, and antioxidant properties of the polysaccharide (RAMP1) from A. macrocephala in Pan'an. These results highlight the potential applications of RAMP1 in the food and biotechnology industries.

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迄今为止，还没有关于磐安白术多糖结构和性质的研究报道。本文首次从磐安白术中分离出一种多糖，命名为 RAMP1。根据化学和核磁共振分析，其结构被确定为由果糖和葡萄糖组成的杂多糖，摩尔比为 20:1，分子量为 3494 Da。扫描电子显微镜分析表明，RAMP1 呈不规则卷曲的光滑片状结构。热分析结果表明，RAMP1 在 165 °C 以下具有很高的热稳定性。流变学研究表明，温度和浓度都会影响 RAMP1 的粘度。RAMP1 溶液的粘度随着剪切速率的增加而降低，表现出剪切稀化行为，表明 RAMP1 溶液是一种非牛顿流体。RAMP1 对 DPPH、超氧化物、ABTS 和羟自由基具有强效的剂量依赖性抗氧化活性，其中对羟自由基的抑制作用最强（8 mg/mL 时为 83.97 ± 1.62%）。本文首次报道了磐安大叶桉多糖（RAMP1）的结构、流变学和抗氧化特性。这些结果凸显了 RAMP1 在食品和生物技术行业的潜在应用。

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".