{"title":"A Multiplex Bead Serology Panel For Vaccine-Preventable Diseases Using Dried Blood Spots.","authors":"Paul D Olivo, Sudip Ghatak, Randolph Rasch","doi":"10.2147/RMHP.S506025","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Vaccines are effective tools to improve public health. The effectiveness of vaccines is, however, dependent on the overall level of protection in a population. Antibodies to vaccine-related antigens are good biomarkers of protection and serosurveillance can help target vaccination programs. An integrated approach to perform serosurveillance on multiple vaccine-preventable diseases (VPDs) has been advocated and would be facilitated by a standardized multiplex immunoassay. In this report, we describe the evaluation of the performance of a multi-lyte bead-based serology panel for 12 VPDs which uses a dried blood spot sample from a finger prick (<i>ImmunoProfile Antibody Test System</i>).</p><p><strong>Methods: </strong>Verification/validation studies were performed at a CLIA-certified clinical laboratory (BioAgilytix Labs, Boston, MA) on blood collected from dried blood spot (DBS) card samples from adults and children. In addition, proof-of-principle pilot serosurveillance studies were performed to demonstrate the potential of this test to identify protection gaps in adult and pediatric populations.</p><p><strong>Results: </strong>This study demonstrates that the ImmunoProfile Antibody Test System has the requisite analytical performance to be a reliable tool for determining levels of protection to VPDs. The pilot serosurveillance studies demonstrate that this test reveals gaps in protection comparable to what has been shown using immunoassays for individual antibodies using serum samples.</p><p><strong>Conclusion: </strong>Serological survey data obtained with the validated ImmunoProfile Antibody Test System could provide a wealth of information on levels of protection and could unearth vaccination gaps that may not have been anticipated.</p>","PeriodicalId":56009,"journal":{"name":"Risk Management and Healthcare Policy","volume":"18 ","pages":"923-932"},"PeriodicalIF":2.0000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11932034/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Risk Management and Healthcare Policy","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/RMHP.S506025","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"HEALTH CARE SCIENCES & SERVICES","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Vaccines are effective tools to improve public health. The effectiveness of vaccines is, however, dependent on the overall level of protection in a population. Antibodies to vaccine-related antigens are good biomarkers of protection and serosurveillance can help target vaccination programs. An integrated approach to perform serosurveillance on multiple vaccine-preventable diseases (VPDs) has been advocated and would be facilitated by a standardized multiplex immunoassay. In this report, we describe the evaluation of the performance of a multi-lyte bead-based serology panel for 12 VPDs which uses a dried blood spot sample from a finger prick (ImmunoProfile Antibody Test System).
Methods: Verification/validation studies were performed at a CLIA-certified clinical laboratory (BioAgilytix Labs, Boston, MA) on blood collected from dried blood spot (DBS) card samples from adults and children. In addition, proof-of-principle pilot serosurveillance studies were performed to demonstrate the potential of this test to identify protection gaps in adult and pediatric populations.
Results: This study demonstrates that the ImmunoProfile Antibody Test System has the requisite analytical performance to be a reliable tool for determining levels of protection to VPDs. The pilot serosurveillance studies demonstrate that this test reveals gaps in protection comparable to what has been shown using immunoassays for individual antibodies using serum samples.
Conclusion: Serological survey data obtained with the validated ImmunoProfile Antibody Test System could provide a wealth of information on levels of protection and could unearth vaccination gaps that may not have been anticipated.
期刊介绍：
Risk Management and Healthcare Policy is an international, peer-reviewed, open access journal focusing on all aspects of public health, policy and preventative measures to promote good health and improve morbidity and mortality in the population. Specific topics covered in the journal include:
Public and community health
Policy and law
Preventative and predictive healthcare
Risk and hazard management
Epidemiology, detection and screening
Lifestyle and diet modification
Vaccination and disease transmission/modification programs
Health and safety and occupational health
Healthcare services provision
Health literacy and education
Advertising and promotion of health issues
Health economic evaluations and resource management
Risk Management and Healthcare Policy focuses on human interventional and observational research. The journal welcomes submitted papers covering original research, clinical and epidemiological studies, reviews and evaluations, guidelines, expert opinion and commentary, and extended reports. Case reports will only be considered if they make a valuable and original contribution to the literature. The journal does not accept study protocols, animal-based or cell line-based studies.
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