Hypoxia-conditioned mesenchymal stem cells (MSC) exosomes attenuate ultraviolet-B (UVB)-mediated malondialdehyde (MDA) and matrix metalloproteinase-1 (MMP)-1 upregulation in collagen loss models.

Q2 Medicine

Medicinski Glasnik Pub Date : 2025-01-01 DOI:10.17392/1923-22-01

Jessica Angelina, Agung Putra, Setyo Trisnadi, Dedy Hermansyah, Eko Setiawan, Titiek Sumarawati, Nur Dina Amalina

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引用次数: 0

Abstract

Aim: To investigate the effects of exosome-derived human mesenchymal stem cells (EH-MSCs) on matrix metalloproteinase-1 (MMP)-1 and malondialdehyde (MDA) levels in ultraviolet-B (UVB)-irradiated Wistar rats.

Methods: The study used a post-test-only control group design with randomized allocation. Thirty rats were exposed to UVB radiation (302 nm, 150 mJ/cm²) for 8 minutes, five times a week over two weeks. Five groups were established: a healthy control (G1), a negative control (G2), a positive control that received a 200 μL subcutaneous injection of hyaluronic acid (HA) (G3), a treatment group 1 that received a 200 μL subcutaneous injection of exosome-derived EH-MSCs (G4), and a treatment group 2 that received a 300 μL subcutaneous injection of EH-MSCs (G5). An ELISA was used to quantify the levels of MDA and MMP-1.

Results: A significant reduction in the mean level of MDA in groups G4 (368.33 ± 59.67) and G5 (329.33 ± 82.06) was noted when compared to the negative control group G2 (686.58 ± 119.01) (p< 0.05). Similarly, MMP-1 showed a significant decrease in mean levels for G4 (0.08 ± 0.04) and G5 (0.07 ± 0.04) compared to G2 (0.33 ± 0.06) (p< 0.05).

Conclusion: These findings suggest that EH-MSCs have potent antioxidant and antiinflammatory properties, mitigating UVB-induced skin damage by reducing oxidative stress markers.

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缺氧条件间充质干细胞（MSC）外泌体减弱紫外线b （UVB）介导的丙二醛（MDA）和基质金属蛋白酶-1 (MMP)-1上调。

目的：研究外泌体衍生的人间充质干细胞（EH-MSCs）对紫外线-B（UVB）照射Wistar大鼠基质金属蛋白酶-1（MMP）-1和丙二醛（MDA）水平的影响：研究采用随机分配的仅试验后对照组设计。30 只大鼠接受紫外线-B 辐射（302 纳米，150 毫焦/厘米²）照射 8 分钟，每周 5 次，持续两周。实验共分五组：健康对照组（G1）、阴性对照组（G2）、皮下注射 200 μL 透明质酸（HA）的阳性对照组（G3）、皮下注射 200 μL 外泌体衍生 EH-MSCs 的治疗组 1（G4）和皮下注射 300 μL EH-MSCs 的治疗组 2（G5）。用酶联免疫吸附法量化 MDA 和 MMP-1 的水平：结果：与阴性对照组 G2（686.58 ± 119.01）相比，G4 组（368.33 ± 59.67）和 G5 组（329.33 ± 82.06）的 MDA 平均水平明显下降（p< 0.05）。同样，与 G2 组（0.33 ± 0.06）相比，G4 组（0.08 ± 0.04）和 G5 组（0.07 ± 0.04）的 MMP-1 平均水平显著下降（p< 0.05）：这些研究结果表明，EH-间充质干细胞具有强大的抗氧化和抗炎特性，可通过减少氧化应激标记物来减轻紫外线诱导的皮肤损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Medicinski Glasnik 医学-医学：内科

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Medicinski Glasnik (MG) is the official publication (two times per year) of the Medical Association of Zenica-Doboj Canton. Manuscripts that present of original basic and applied research from all fields of medicine (general and clinical practice, and basic medical sciences) are invited.