Quantification of the immunohistochemical staining of fibroblast activation protein in intrathoracic solitary fibrous tumors using QuPath.

IF 1.6 4区医学 Q2 SURGERY

Surgery Today Pub Date : 2025-09-01 Epub Date: 2025-03-24 DOI:10.1007/s00595-025-03024-y

Akiisa Omura, Toru Kimura, Tomohiro Maniwa, Tadashi Watabe, Keiichiro Honma, Yasushi Shintani, Jiro Okami

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引用次数: 0

Abstract

Purpose: Solitary fibrous tumors (SFTs) are rare mesenchymal neoplasms that can develop in the pleura. In the past, SFTs were considered benign, but there have been reports of SFTs being highly malignant. Fibroblast activation protein (FAP) is a serine protease, overexpressed in various cancers, which has been explored as a diagnostic and therapeutic target. We analyzed patients who underwent resection of an intrathoracic SFT, including metastatic pulmonary nodules from extrathoracic organs.

Methods: The subjects of this retrospective study were seven patients with a primary SFT and two with metastatic SFTs in the lungs. After immunohistochemical staining of the resected tumors, quantification of the stained area was performed using QuPath.

Results: Immunohistochemical quantification of FAP showed that it was expressed to varying degrees in the intrathoracic SFTs, with higher expression levels observed in metastatic SFTs than in primary pleural SFTs. Pathological examination confirmed the expression of FAP.

Conclusion: Our results support the potential usefulness of FAP in the diagnosis of intrathoracic SFTs, including metastatic pulmonary nodules.

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QuPath定量定量胸内孤立性纤维性肿瘤成纤维细胞活化蛋白免疫组化染色。

目的：孤立性纤维性肿瘤（SFTs）是一种罕见的发生于胸膜的间充质肿瘤。过去，SFTs被认为是良性的，但也有报道称SFTs是高度恶性的。成纤维细胞活化蛋白（FAP）是一种丝氨酸蛋白酶，在多种癌症中过表达，已被探索作为诊断和治疗靶点。我们分析了接受胸内SFT切除术的患者，包括来自胸外器官的转移性肺结节。方法：本回顾性研究的对象是7例原发性SFT患者和2例肺转移性SFT患者。切除肿瘤免疫组化染色后，使用QuPath对染色面积进行定量。结果：免疫组化定量显示FAP在胸内SFTs中有不同程度的表达，转移性SFTs中的表达水平高于原发性胸膜SFTs。病理检查证实FAP表达。结论：我们的研究结果支持FAP在诊断胸内SFTs（包括转移性肺结节）中的潜在作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Surgery Today 医学-外科

CiteScore

4.90

自引率

4.00%

发文量

208

审稿时长

1 months

期刊介绍： Surgery Today is the official journal of the Japan Surgical Society. The main purpose of the journal is to provide a place for the publication of high-quality papers documenting recent advances and new developments in all fields of surgery, both clinical and experimental. The journal welcomes original papers, review articles, and short communications, as well as short technical reports("How to do it"). The "How to do it" section will includes short articles on methods or techniques recommended for practical surgery. Papers submitted to the journal are reviewed by an international editorial board. Field of interest: All fields of surgery.