A rapid and efficient in vivo inoculation method for introducing tree stem canker pathogens onto leaves: suitable for large-scale assessment of resistance in poplar breeding progeny.
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引用次数: 0
Abstract
Background: Hybrid breeding, a direct and efficient strategy for disease control and management in tree species, is currently limited by the selection method of resist clones: the "in vitro stem segment inoculation method". This method, constrained by the availability of inoculating materials, cannot rapidly, efficiently, and cost-effectively screen the resistance of all hybrid clones. To overcome these limitations, we introduce a novel pathogen inoculation method for the resistance assessment of hybrid clones in the poplar-Valsa sordida pathosystem. This method involves inoculating the stem canker pathogen on the host leaf, a unique and promising approach we have successfully validated.
Results: Results showed that stem canker pathogen V. sordida induced the extended necrotic lesion and even induced the formation of pycnidium structure and conidia on the leaf surface 5 days after mycelium inoculation; (1) the upper 5-7thleaves exhibited higher resistance than the middle 18-20th leaves; (2) the shading conditions induced more severe symptoms on the leaves than lighting conditions; (3) the poplar leaves were more susceptible to the juvenile mycelium inoculums (4-day-cultured) than the old ones (7-day-cultured). Our results demonstrate the robustness ofthe "in vivo leaf inoculation method" in revealing the resistance differentiation in poplar hybrid clones. According to the leaf necrotic area disease index, we divided these poplar clones into seven different resistance groups. The resistance assessed by leaf assessment was validated in 15 selected poplar clones using the "in vitro stem segment inoculation method". Results showed that the effectiveness of these two methods was consistent. Moreover, results also revealed the pathogenicity diversity of the pathogen population of tree species using leaf the inoculation method.
Conclusions: Compared to the conventional "in vitro stem segment inoculation method", the leaf method has the advantages of abundant inoculation materials, easy operation, rapid disease onset, and almost no adverse effect on the host. It is particularly suitable for the resistance screening of all progeny and the early (seedling) phenotypic selection of resistant poplar clones in poplar stem disease resistance breeding. The "in vivo leaf inoculation method" holds significant promise in poplar breeding, tree pathology, and molecular biology research on tree stem diseases.
期刊介绍：
Plant Methods is an open access, peer-reviewed, online journal for the plant research community that encompasses all aspects of technological innovation in the plant sciences.
There is no doubt that we have entered an exciting new era in plant biology. The completion of the Arabidopsis genome sequence, and the rapid progress being made in other plant genomics projects are providing unparalleled opportunities for progress in all areas of plant science. Nevertheless, enormous challenges lie ahead if we are to understand the function of every gene in the genome, and how the individual parts work together to make the whole organism. Achieving these goals will require an unprecedented collaborative effort, combining high-throughput, system-wide technologies with more focused approaches that integrate traditional disciplines such as cell biology, biochemistry and molecular genetics.
Technological innovation is probably the most important catalyst for progress in any scientific discipline. Plant Methods’ goal is to stimulate the development and adoption of new and improved techniques and research tools and, where appropriate, to promote consistency of methodologies for better integration of data from different laboratories.
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