Genome-wide analysis of ascorbate peroxidase and functional characterization of SpAPX249b and SpAPX285c for salt tolerance in Sesuvium portulacastrum L.

Abstract

Key message: We have identified 33 SpAPXs from S. portulacastrum genome and found SpAPX249b and SpAPX285c are important for halophyte salt tolerance. Ascorbate peroxidase (APX) is a vital antioxidant enzyme, involved in plant development and stress response by scavenging excessive reactive oxygen species (ROS). APX genes have been characterized in many plant species. However, their role in Sesuvium portulacastrum L. has not yet to be fully investigated. Here, we identified 33 SpAPXs from its genome and divided them into five subgroups across the 16 chromosomes. Cis-element analysis of their promoters indicated that all the detected SpAPXs showed potential roles in response to biotic and abiotic stresses as well as phytohormone effects on the plant growth and development. Transcriptomic data of the different tissues revealed that 9 SpAPX genes were specifically expressed in root and 13 ones were specifically expressed in leaves, with SpAPX249b prominently expressed in root and SpAPX285c in leaves. Moreover, quantitative real-time PCR analysis revealed that both SpAPX249b and SpAPX285c genes expressed only after NaCl application and were sharply induced in the high concentration of NaCl treatments. Our findings suggested that SpAPX249b and SpAPX285c may associate with plant salt tolerance and can serve as valuable genes for enhancing salt tolerance in other plants. By introducing these genes into other plants, it is possible to develop new varieties of salt-tolerant crops, thereby expanding the utilization of saline-alkali land and increasing agricultural productivity. In coastal saline-alkali wetlands, this halophyte can thrive in large numbers due to its inherent salt-tolerant genes, contributing to the restoration of polluted or ecologically degraded coastal saline-alkali wetlands.