Overexpression of lncRNA22524 from Dongxiang Wild Rice Reduces Drought and Salt Stress Tolerance in Cultivated Rice.

IF 4.8 1区 农林科学 Q1 AGRONOMY
Rice Pub Date : 2025-03-25 DOI:10.1186/s12284-025-00777-5
Yong Chen, Yingying Mao, Hong Xie, Xinjian Zou, Wanling Yang, Rifang Gao, Jiankun Xie, Fantao Zhang
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Abstract

Drought and salt stresses are major challenges to rice production, and a deep understanding of the mechanisms for tolerance could help deal with the challenges. Long non-coding RNAs (lncRNAs) play crucial roles in gene regulation. Previously, lncRNA22524 has been identified as a drought stress-responsive lncRNA from Dongxiang wild rice (DXWR). Nevertheless, its reactions to abiotic stresses in genetics and physiology remained unclear. In this study, we employed a rapid amplification of cDNA ends (RACE) to obtain the full-length cDNA of lncRNA22524 from DXWR, analyzed its cellular localization, built an overexpression vector to generate transgenic lines of cultivated rice and evaluated its impact in genetics and physiology. After treated with drought and salt stress, the overexpressed lines exhibited much more injuries and lower rates of survival, more reactive oxygen species (ROS) and malondialdehyde (MDA), lower antioxidant enzymes and lower proline (Pro) and soluble sugar (SS) than their wild-type (WT). Furthermore, transcriptome analysis of overexpressed lines with weaker tolerance than WT revealed 1,233 differentially expressed genes (DEGs), where most DEGs were involved in phenylpropanoid biosynthesis, photosynthesis and glutathione metabolism. These findings demonstrated that lncRNA22524 negatively regulated rice responses to drought and salt stress, which clear way of working from transcription to metabolic products should be worth of further study.

东乡野生稻lncRNA22524过表达降低栽培稻抗旱性和耐盐性
干旱和盐胁迫是水稻生产面临的主要挑战,深入了解耐受性机制有助于应对这些挑战。长链非编码rna (lncRNAs)在基因调控中起着至关重要的作用。此前,lncRNA22524已被鉴定为来自东乡野生稻(DXWR)的干旱胁迫响应lncRNA。然而,其对非生物胁迫在遗传学和生理学上的反应尚不清楚。本研究采用cDNA末端快速扩增技术(RACE)从DXWR中获得lncRNA22524的全长cDNA,分析其细胞定位,构建过表达载体生成栽培水稻转基因系,并评价其在遗传学和生理学上的影响。干旱和盐胁迫处理后,过表达品系比野生型(WT)损伤更严重,存活率更低,活性氧(ROS)和丙二醛(MDA)含量更高,抗氧化酶含量更低,脯氨酸(Pro)和可溶性糖(SS)含量更低。此外,对抗性较弱的过表达品系进行转录组分析,发现了1233个差异表达基因(DEGs),其中大多数差异表达基因与苯丙素生物合成、光合作用和谷胱甘肽代谢有关。这些发现表明lncRNA22524负调控水稻对干旱和盐胁迫的响应,明确了其从转录到代谢产物的工作方式值得进一步研究。
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来源期刊
Rice
Rice AGRONOMY-
CiteScore
10.10
自引率
3.60%
发文量
60
审稿时长
>12 weeks
期刊介绍: Rice aims to fill a glaring void in basic and applied plant science journal publishing. This journal is the world''s only high-quality serial publication for reporting current advances in rice genetics, structural and functional genomics, comparative genomics, molecular biology and physiology, molecular breeding and comparative biology. Rice welcomes review articles and original papers in all of the aforementioned areas and serves as the primary source of newly published information for researchers and students in rice and related research.
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