Marivivens marinum sp. nov., isolated from tidal flat, Gochang, South Korea.

Abstract

A Gram-negative, rod-shaped, non-motile, aerobic bacterium, designated MK-3^T, was isolated from shallow seawater in Gochang, Republic of Korea. Growth of strain MK-3^T occurred at 15-40 °C (30 °C), pH 6.0-7.0 (pH 7.0) and in the presence of 2-3 % NaCl (2%). Phylogenetic analysis based on the 16S rRNA gene sequence placed strain MK-3^T within the family Roseobacteraceae. It exhibited sequence similarities of 95.7% with Salipiger marinus CK-I3-6^T, Salipiger aestuarii DSM 22011^T and Salipiger pentaromativorans P9^T; 95.4% with Ruegeria alba 1NDH52C^T, Salipiger manganoxidans VSW210^T and Salipiger thiooxidans DSM 10146^T; 95.3% with Marivivens donghaensis AM-4^T and Histidinibacterium lentulum B17^T; and 95.2% with Marivivens geojensis FJ12^T. Phylogenetic and phylogenomic analyses consistently demonstrated that strain MK-3^T formed a distinct lineage within the genus Marivivens, clustering with its closest relatives. The major fatty acids were C_18 : 1  ω7c/C1_8 : 1  ω6c, C_18 : 1  ω7c 11-methyl and C_16 : 0. The genome length of strain MK-3^T was 3.3 Mbp, and the DNA G+C content was 62.8 mol%. The strain contained Q-10 as the major ubiquinone. The polar lipids consisted of three phosphatidylinositol mannosides and a diphosphatidylglycerol. Based on its phenotypic, chemotaxonomic, phylogenetic and genomic characteristics, strain MK-3^T represents a novel species in the genus Marivivens, for which the name Marivivens marinum sp. nov. is proposed. The type strain is MK-3^T (=KEMB 21417^T=KCTC 8294^T=JCM 36630^T).