Zoonotic transmission of the Mycobacterium tuberculosis complex between cattle and humans in Central Ethiopia.

IF 2.6 2区农林科学 Q1 VETERINARY SCIENCES

Frontiers in Veterinary Science Pub Date : 2025-03-10 eCollection Date: 2025-01-01 DOI:10.3389/fvets.2025.1527279

Tefera Woldemariam, Temesgen Mohammed, Aboma Zewude, Mahlet Chanyalew, Hazim O Khalifa, Gezahegne Mamo, Gobena Ameni

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引用次数: 0

Abstract

Introduction: The zoonotic transmission of tuberculosis (TB) from cattle to humans has long been recognized, while the reverse transmission from humans to animals has only recently been reported. The socioeconomic conditions in rural Ethiopia are conducive to the zoonotic and reverse zoonotic transmission of TB between cattle and humans. This study aimed to explore the transmission of the Mycobacterium tuberculosis complex between cattle and humans in Central Ethiopia.

Methods: To achieve this objective, a cross-sectional study was conducted involving 1,896 cattle slaughtered at two abattoirs and 392 human subjects suspected of tuberculosis who visited health institutions for treatment. Mycobacteriological culture and spoligotyping were used for the study. Additionally, the Spoligotype International Types and VNTR (variable number of DNA tandem repeats) International Types (SITVIT2) database and the online tool "Run TB-Lineage" were used to identify SIT and lineages of the isolates from both humans and cattle.

Results: Culture positivity was found in 26.3% (21/80) of the gross TB-suspicious tissue lesions in the lungs and lymph nodes (mandibular, retropharyngeal, cranial, and caudal mediastinal, as well as left and right bronchial, hepatic, and mesenteric lymph nodes) of cattle. Of the 21 cattle isolates, 12 (57.2%) were identified as M. bovis, while the remaining 9 (42.8%) were classified as M. tuberculosis. Similarly, only 22% (86/392) of the sputum samples from TB-suspicious humans were culture positive. These 86 human isolates included 81 M. tuberculosis, three M. africanum, and two M. bovis, as determined by spoligotyping. SIT50, SIT118, and SIT1318, which belong to the human species, were isolated from both humans and cattle. The two human M. bovis isolates exhibited the pattern of SB1443, which was not identified in cattle within this study area.

Conclusion: The zoonotic and reverse zoonotic transmissions of TB were confirmed in Ethiopia by isolating two M. bovis from humans and nine M. tuberculosis from cattle, which suggested a greater role for M. tuberculosis in cattle compared to M. bovis in humans.

查看原文本刊更多论文

埃塞俄比亚中部牛与人之间结核分枝杆菌复合体的人畜共患传播。

前言：很早以前人们就认识到牛向人类传播结核病（TB）的人畜共患传播，而人向动物的反向传播直到最近才有报道。埃塞俄比亚农村的社会经济条件有利于牛与人之间的人畜共患和反向人畜共患结核病传播。本研究旨在探讨埃塞俄比亚中部牛与人之间结核分枝杆菌复合体的传播。方法：为了实现这一目标，对两个屠宰场屠宰的1896头牛和392名前往卫生机构接受治疗的疑似结核病患者进行了横断面研究。分枝杆菌培养和孢子分型被用于研究。此外，利用Spoligotype International Types和VNTR（可变数量的DNA串联重复序列）International Types （SITVIT2）数据库和Run TB-Lineage在线工具对人类和牛分离物的SIT和谱系进行鉴定。结果：牛肺和淋巴结（下颌、咽后、颅、尾纵隔以及左右支气管、肝脏和肠系膜淋巴结）的大体结核可疑组织病变中培养阳性的比例为26.3%（21/80）。21株分离株中，12株（57.2%）鉴定为牛分枝杆菌，其余9株（42.8%）鉴定为结核分枝杆菌。同样，结核可疑人群的痰样本中只有22%（86/392）培养阳性。这86株人类分离株包括81株结核分枝杆菌、3株非洲分枝杆菌和2株牛分枝杆菌。SIT50、SIT118和SIT1318分别从人类和牛中分离得到，属于人类物种。这两株人牛分枝杆菌分离株具有SB1443型，在本研究区域的牛中未发现SB1443型。结论：在埃塞俄比亚通过从人身上分离出2例牛分枝杆菌和从牛身上分离出9例结核分枝杆菌，证实了结核的人畜共患和反向人畜共患传播，这表明牛分枝杆菌的作用比人的牛分枝杆菌更大。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Veterinary Science Veterinary-General Veterinary

CiteScore

4.80

自引率

9.40%

发文量

1870

审稿时长

14 weeks

期刊介绍： Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy. Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field. Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.