The preventive effect of probiotic Lactobacillus plantarum X86 isolated from raw milk on Staphylococcus aureus-induced mastitis in rats.

IF 2.6 2区农林科学 Q1 VETERINARY SCIENCES

Frontiers in Veterinary Science Pub Date : 2025-03-10 eCollection Date: 2025-01-01 DOI:10.3389/fvets.2025.1476232

Xiulan Xie, Mei Cao, Shiying Yan, Haihui Gao, Yuwei Yang, Jiayi Zeng, Gang Zhang, Jian Zhao

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Abstract

Mastitis is the most common and challenging disease that affects dairy animal welfare and causes huge economic loss in dairy industry globally. Conventional antibiotic treatment of mastitis raised the drug resistance and unsuccessful therapy. As an alternative approach, probiotic lactobacilli had shown multifunctional effects against diseases. Lactobacillus strains against mastitis are worth screening and evaluating. In this study, milk-derived Lactobacillus spp. from Ningxia, China were screened in vitro and the anti-mastitis effect of a candidate strain was evaluated through a Staphylococcus aureus-induced rat mastitis model. The results showed that Lactobacillus plantarum X86 exhibited a high adhesion rate of MAC-T cells, presented the best probiotic properties, and demonstrated anti-S. aureus effects in vitro through comprehensive assessment. Furthermore, L. plantarum X86 alleviated pathological damage to the mammary gland, liver, and colon, inhibited the mRNA expression of pro-inflammatory cytokines factors IL-1β, IL-6, and TNF-α in mammary gland tissue; and increased the content of intestine SCFAs in a rat mastitis model induced by S. aureus. In conclusion, our results suggested that L. plantarum X86 could be a promising probiotic for the prevention and treatment of S. aureus-induced mastitis.

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乳腺炎是最常见、最具挑战性的疾病，它影响着乳牛的福利，并给全球乳业造成巨大的经济损失。传统的抗生素治疗乳腺炎会产生耐药性，治疗效果不佳。作为一种替代方法，益生菌乳酸杆菌对疾病具有多重功效。针对乳腺炎的乳酸杆菌菌株值得筛选和评估。本研究对来自中国宁夏的乳源性乳酸杆菌进行了体外筛选，并通过金黄色葡萄球菌诱导的大鼠乳腺炎模型评估了候选菌株的抗乳腺炎效果。结果表明，植物乳杆菌 X86 对 MAC-T 细胞具有较高的粘附率，益生性能最佳，并通过综合评估在体外表现出抗金黄色葡萄球菌的效果。此外，植物乳杆菌 X86 还能减轻乳腺、肝脏和结肠的病理损伤，抑制乳腺组织中促炎细胞因子 IL-1β、IL-6 和 TNF-α 的 mRNA 表达，并增加金黄色葡萄球菌诱导的大鼠乳腺炎模型中肠道 SCFAs 的含量。总之，我们的研究结果表明，植物乳杆菌 X86 是一种很有前景的益生菌，可用于预防和治疗金黄色葡萄球菌诱发的乳腺炎。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Veterinary Science Veterinary-General Veterinary

CiteScore

4.80

自引率

9.40%

发文量

1870

审稿时长

14 weeks

期刊介绍： Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy. Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field. Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.