Phytochemical Insights and Acetylcholinesterase-Targeted Nematicidal Activity of Digitalis purpurea L. Extracts in Biological Control of Meloidogyne incognita.

IF 2.3 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry & Biodiversity Pub Date : 2025-03-25 DOI:10.1002/cbdv.202403274

Himani Karakoti, Ravendra Kumar, Om Prakasha, Satya Kumar, D S Rawat, Mozaniel de Oliveira

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引用次数: 0

Abstract

Digitalis purpurea L. is traditionally valued for its cardiac glycosides, but its pesticidal properties remain underexplored. This study evaluated its nematicidal efficacy against Meloidogyne incognita, focusing on acetylcholinesterase (AChE) inhibition as a potential mechanism. Extracts obtained using Soxhlet extraction with hexane (DPHE), dichloromethane (DPDE), and methanol (DPME) were analyzed via GC-MS. DPHE contained fatty acids, including hexadecenoic acid (20.38%) and linoleic acid (14.43%). DPDE was rich in bioactives like gitogenin (8.89%) and digitoxigenin (6.8%). DPME featured β-D-glucopyranose, 1,6-anhydro (15.55%) and smilagenin (5.49%). Nematicidal assays showed DPDE achieved 100% mortality at 1000 µg/mL and 91.23% egg hatching inhibition. It also demonstrated 87.88% AChE inhibition, surpassing physostigmine (81.75%). Molecular docking revealed strong interactions between DPDE compounds and AChE. These findings suggest D. purpurea, particularly its dichloromethane extract, as a promising eco-friendly nematicidal agent.

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来源期刊

Chemistry & Biodiversity 环境科学-化学综合

CiteScore

3.40

自引率

10.30%

发文量

475

审稿时长

2.6 months

期刊介绍： Chemistry & Biodiversity serves as a high-quality publishing forum covering a wide range of biorelevant topics for a truly international audience. This journal publishes both field-specific and interdisciplinary contributions on all aspects of biologically relevant chemistry research in the form of full-length original papers, short communications, invited reviews, and commentaries. It covers all research fields straddling the border between the chemical and biological sciences, with the ultimate goal of broadening our understanding of how nature works at a molecular level. Since 2017, Chemistry & Biodiversity is published in an online-only format.