Genetic diversity of true morels (Morchella) in Pakistan inferred from multilocus DNA sequence data augmented by a retrospective analysis of Pakistani and Indian morel sequences in GenBank.

IF 2.6 2区 生物学 Q2 MYCOLOGY
Surat Un Nisa, Kerry O'Donnell, Hussain Badshah, Sayed Afzal Shah, Abdul Samad Mumtaz
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Abstract

The current study was conducted to obtain the first detailed assessment of true morel (Morchella) species diversity in Pakistan. A collection of 179 morels was obtained by conducting field surveys in 13 districts during spring and autumn of 2014 through 2020. Species were identified phylogenetically by analyzing portions of two (RPB1, RPB2) or four (RPB1, RPB2, TEF1, ITS rDNA) marker loci together with authentic reference sequences of known species and by conducting BLASTn queries of National Center for Biotechnology Information (NCBI) GenBank. A retrospective analysis of morel sequences from Pakistani (N = 52) and Indian (N = 69) collections accessioned in GenBank was also conducted to expand and compare our knowledge of Morchella species diversity in both countries. Analyses of our 179-morel collection and the 52 Pakistani sequences deposited in GenBank indicated that at least 20 species are present in Pakistan, including five putatively novel taxa within the Elata subclade here informally distinguished as Morchella spp. (Mel-43 to Mel-47). Phylospecies Mel-45 and Mel-46 were described formally as M. pycnogranulata and M. waziristanica, respectively. By comparison, analyses of the 69 Indian sequences deposited in GenBank indicated that at least 12 species are extant in India. However, only 4/9 Pakistani and 3/12 Indian species were correctly identified in GenBank. Two collections of the steppe morel, M. steppicola (Mes-1), from Afghanistan were also included in the present study, which extends the southeasternmost range of this morphologically distinct species.

通过对GenBank中巴基斯坦和印度羊肚菌序列的回顾性分析,从多位点DNA序列数据推断巴基斯坦真正羊肚菌(羊肚菌)的遗传多样性。
本研究是为了获得巴基斯坦真正的羊肚菌(羊肚菌)物种多样性的首次详细评估。2014年春季和秋季至2020年期间,通过在13个地区进行实地调查,收集了179株鼹鼠。通过分析2个(RPB1、RPB2)或4个(RPB1、RPB2、TEF1、ITS rDNA)标记位点的部分片段和已知物种的可靠参考序列,并通过国家生物技术信息中心(NCBI) GenBank的BLASTn查询进行物种系统发育鉴定。我们还对基因库中巴基斯坦(N = 52)和印度(N = 69)收集的羊肚菌序列进行了回顾性分析,以扩大和比较我们对两国羊肚菌物种多样性的了解。对我们收集的179个羊肚菌和存放在GenBank中的52个巴基斯坦序列的分析表明,巴基斯坦至少有20个物种,其中包括Elata亚支中的5个假定的新分类群,在这里被非正式地称为羊肚菌属(Mel-43至Mel-47)。系统种Mel-45和Mel-46分别被正式描述为M. pycnogranulata和M. waziristanica。通过对GenBank中69个印度序列的比较分析表明,印度至少存在12个物种。然而,只有4/9的巴基斯坦和3/12的印度物种在GenBank中被正确识别。来自阿富汗的两组草原鼹鼠M. steppicola (Mes-1)也包括在本研究中,这扩大了这种形态上独特的物种的最东南范围。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Mycologia
Mycologia 生物-真菌学
CiteScore
6.20
自引率
3.60%
发文量
56
审稿时长
4-8 weeks
期刊介绍: International in coverage, Mycologia presents recent advances in mycology, emphasizing all aspects of the biology of Fungi and fungus-like organisms, including Lichens, Oomycetes and Slime Molds. The Journal emphasizes subjects including applied biology, biochemistry, cell biology, development, ecology, evolution, genetics, genomics, molecular biology, morphology, new techniques, animal or plant pathology, phylogenetics, physiology, aspects of secondary metabolism, systematics, and ultrastructure. In addition to research articles, reviews and short notes, Mycologia also includes invited papers based on presentations from the Annual Conference of the Mycological Society of America, such as Karling Lectures or Presidential Addresses.
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