The impact of in ovo injection of cluster bean peptide on hatchability, growth performance, carcass characteristics, digestive enzymes, and blood indices of broiler chickens.

Abstract

The administration of bioactive short peptides through in ovo feeding can improve the overall health and performance of broiler chickens for the poultry industry. Additionally, bioactive peptides possess biological features that have the potential to be beneficial in preventing many metabolic illnesses; hence, the ingestion of these peptides holds the potential to be advantageous for human health. In light of this, the current work aimed to study the impacts of in ovo feeding during the late stages of embryonic development with cluster bean peptide (CBP) on the hatchability, productive performance, lipid profile, liver and kidney functions, immunological response, and antioxidant status of broilers. Six hundred and forty-eight (648) fertilized Ross 308 broiler breeder eggs were used in this study. To remove infertile eggs and dead embryos, the eggs were manually candled on 7 and 17 day of incubation (DOI). On the 18.5th DOI, the eggs were separated into four treatment groups (156 eggs/each), and the first group did not receive any treatment and represented the negative control (NC). Meanwhile, the other treatment groups were injected into the amnion membrane. The second group was only subjected to needle penetration and represented the positive control (PC). The third group was denoted by the letter T1 and received an injection of 1 mg CBP/egg. The fourth group was denoted by the letter T2 and received an injection of 2 mg CBP/egg. In ovo feeding by CBP exhibited significant improvements in the body weight of newly hatched chicks, particularly at the 2 mg CBP level. The administration with CBP did not significantly affect the carcass characteristics of 28-day-old broilers. In ovo-administrated groups with CBP, higher plasma concentrations of total protein and its fractions were observed at hatch and on day 28 of age. In ovo treatment with CBP, blood lipid profile parameters significantly improved at hatch and 28 days of age. Liver and kidney function parameters were improved in response to the in ovo administration with CBP in newly hatched chicks and on day 28 of age. Blood levels of glutathione (GSH) and superoxide dismutase (SOD) were considerably higher in the in ovo-administered groups with CBP; while levels of malondialdehyde (MDA) were significantly reduced due to CBP administration. The activity of digestive enzymes in blood plasma was decreased in newly hatched chicks but increased in 28-day-old broilers in response to in ovo administration with CBP. There was an improvement in the immunological response of hatched chicks from groups injected with CBP, particularly the T2 group (2 mg CBP), as evidenced by increased IgM and IgG levels. The findings presented here indicate that the in ovo administration with CBP, specifically at a dosage of 2 mg, improved growth performance and immune and antioxidant functions.