Acupuncture regulates α-synuclein expression via serping1 in an MPTP-induced mouse model of Parkinsonism.

IF 2.4 3区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE
Acupuncture in Medicine Pub Date : 2025-04-01 Epub Date: 2025-03-25 DOI:10.1177/09645284251327195
Min Hyung Seo, Sujung Yeo
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引用次数: 0

Abstract

Background: Parkinson's disease (PD) is a neurodegenerative disorder that is characterized by a substantial loss of dopaminergic cells in the substantia nigra (SN) and the formation of intracellular Lewy bodies, which are mainly composed of α-synuclein (α-syn). Acupuncture has been used to improve the symptoms of PD in humans and exhibits a neuroprotective effect against Parkinsonism in animal models. We further investigated the neuroprotective effect of acupuncture via its effect on α-syn levels, dopaminergic cell death and serping1 expression in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of chronic PD.

Methods: Mice were divided into a control group receiving phosphate-buffered saline (CTL) and three model groups receiving MPTP that either remained untreated (MPTP) received verum acupuncture at GB34 and LR3 (MPTP_A) or received control acupuncture at sites not corresponding to any traditional acupuncture point location (MPTP_NA). The signal intensity of serping1 gene expression was assessed using microarray, and α-syn level and dopaminergic cell death were measured by Western blotting, immunohistochemistry and immunofluorescence. To further investigate the relationship between expression of serping1 and α-syn, a cell culture experiment was carried out in a 1-methyl-4-phenylpyridinium (MPP+) treated neuroblastoma cell line (SH-SY5Y) with and without serping1 knockdown using short interfering (si)RNA.

Results: Acupuncture at GB34 and LR3 attenuated the MPTP-induced decrease in tyrosine hydroxylase (TH) levels and increase in α-syn levels in the SN. Furthermore, verum acupuncture prevented the increase in serping1 level induced by MPTP. In SH-SY5Y cells, MPP+ treatment increased α-syn and decreased both TH expression and cell viability; however, these effects were mitigated by serping1 knockdown.

Conclusions: Our results suggest that an MPTP-induced reduction in serping1 level leads to decreased TH and increased α-syn expression, and that these effects can be attenuated/blocked by acupuncture at GB34 and LR3. Our findings provide new evidence for the neuroprotective effects of acupuncture on dopaminergic cells, which may be mediated by control of serping1 expression.

针刺通过serping1调节mptp诱导的帕金森小鼠模型中α-突触核蛋白的表达。
背景:帕金森病(PD)是一种神经退行性疾病,其特征是黑质(SN)内多巴胺能细胞大量丧失,细胞内形成主要由α-突触核蛋白(α-syn)组成的路易小体。针灸已被用于改善人类帕金森病的症状,并在动物模型中显示出对帕金森病的神经保护作用。在1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的慢性PD小鼠模型中,我们进一步通过针刺对α-syn水平、多巴胺能细胞死亡和serping1表达的影响来研究针刺的神经保护作用。方法:将小鼠分为磷酸缓冲盐水组(CTL)和MPTP模型组(MPTP),分别在未治疗的情况下(MPTP)在GB34和LR3部位进行verum针刺(MPTP_A)或在与任何传统穴位位置不对应的部位进行对照针刺(MPTP_NA)。采用芯片检测serping1基因表达信号强度,Western blot、免疫组织化学和免疫荧光检测α-syn水平和多巴胺能细胞死亡情况。为了进一步研究serping1与α-syn表达的关系,我们用短干扰RNA (si)对1-甲基-4-苯基吡啶(MPP+)处理的神经母细胞瘤细胞株(SH-SY5Y)进行了serping1敲除和不敲除的细胞培养实验。结果:针刺GB34和LR3可减轻mptp诱导的SN中酪氨酸羟化酶(TH)水平下降和α-syn水平升高。针刺verum可以抑制MPTP诱导的serping1水平升高。在SH-SY5Y细胞中,MPP+处理增加α-syn,降低TH表达和细胞活力;然而,这些影响被serping1敲低所减轻。结论:mptp诱导的serping1水平降低可导致TH降低和α-syn表达增加,针刺GB34和LR3可减弱或阻断这些作用。我们的研究结果为针灸对多巴胺能细胞的神经保护作用提供了新的证据,这可能是通过控制serping1的表达来介导的。
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来源期刊
Acupuncture in Medicine
Acupuncture in Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
4.70
自引率
4.00%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Acupuncture in Medicine aims to promote the scientific understanding of acupuncture and related treatments by publishing scientific investigations of their effectiveness and modes of action as well as articles on their use in health services and clinical practice. Acupuncture in Medicine uses the Western understanding of neurophysiology and anatomy to interpret the effects of acupuncture.
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