Assessment of Nucleic Acid Quality in Unstained Cytology Specimens for Cancer Genomic Testing.

IF 1.6 4区医学 Q3 PATHOLOGY

Acta Cytologica Pub Date : 2025-03-24 DOI:10.1159/000545423

Hiroya Niimi, Takafumi Onishi, Shoma Nomura, Aya Kumazaki, Yuto Masaki, Hirokazu Odashima, Yukihiko Osawa, Manabu Hattori

{"title":"Assessment of Nucleic Acid Quality in Unstained Cytology Specimens for Cancer Genomic Testing.","authors":"Hiroya Niimi, Takafumi Onishi, Shoma Nomura, Aya Kumazaki, Yuto Masaki, Hirokazu Odashima, Yukihiko Osawa, Manabu Hattori","doi":"10.1159/000545423","DOIUrl":null,"url":null,"abstract":"Introduction: The use of cytological specimens in cancer genome medicine has garnered considerable attention, but the long-term quality of nucleic acids from unstained specimens remains unclear. This study aimed to evaluate the quality of nucleic acids extracted from unstained specimens fixed with 95% ethanol or spray fixation over varying durations.Methods: Two lung cancer cell lines were prepared using the auto smear method and fixed with 95% ethanol, and spray-fixed specimens were stored for 30 min, 1 day, 3 days, 1 week, 2 weeks, 1 month, 3 months, and 6 months. DNA was extracted using a DNA extraction kit, and quality was assessed using agarose gel electrophoresis and PCR.Results: Nucleic acids extracted from unstained specimens showed no fragmentation after six months of fixation and were amplifiable by PCR, regardless of the fixation method.Conclusion: Nucleic acids extracted from unstained specimens preserved high quality over six months, suggesting that such specimens are suitable for genetic testing. This finding has significant implications for the long-term storage and clinical application of cytological specimens in cancer genome medicine.","PeriodicalId":6959,"journal":{"name":"Acta Cytologica","volume":" ","pages":"1-12"},"PeriodicalIF":1.6000,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Cytologica","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1159/000545423","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PATHOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: The use of cytological specimens in cancer genome medicine has garnered considerable attention, but the long-term quality of nucleic acids from unstained specimens remains unclear. This study aimed to evaluate the quality of nucleic acids extracted from unstained specimens fixed with 95% ethanol or spray fixation over varying durations.

Methods: Two lung cancer cell lines were prepared using the auto smear method and fixed with 95% ethanol, and spray-fixed specimens were stored for 30 min, 1 day, 3 days, 1 week, 2 weeks, 1 month, 3 months, and 6 months. DNA was extracted using a DNA extraction kit, and quality was assessed using agarose gel electrophoresis and PCR.

Results: Nucleic acids extracted from unstained specimens showed no fragmentation after six months of fixation and were amplifiable by PCR, regardless of the fixation method.

Conclusion: Nucleic acids extracted from unstained specimens preserved high quality over six months, suggesting that such specimens are suitable for genetic testing. This finding has significant implications for the long-term storage and clinical application of cytological specimens in cancer genome medicine.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Acta Cytologica 生物-病理学

CiteScore

3.70

自引率

11.10%

发文量

审稿时长

4-8 weeks

期刊介绍： With articles offering an excellent balance between clinical cytology and cytopathology, ''Acta Cytologica'' fosters the understanding of the pathogenetic mechanisms behind cytomorphology and thus facilitates the translation of frontline research into clinical practice. As the official journal of the International Academy of Cytology and affiliated to over 50 national cytology societies around the world, ''Acta Cytologica'' evaluates new and existing diagnostic applications of scientific advances as well as their clinical correlations. Original papers, review articles, meta-analyses, novel insights from clinical practice, and letters to the editor cover topics from diagnostic cytopathology, gynecologic and non-gynecologic cytopathology to fine needle aspiration, molecular techniques and their diagnostic applications. As the perfect reference for practical use, ''Acta Cytologica'' addresses a multidisciplinary audience practicing clinical cytopathology, cell biology, oncology, interventional radiology, otorhinolaryngology, gastroenterology, urology, pulmonology and preventive medicine.