Environmental DNA Metabarcoding Elucidates Freshwater Mussel Diversity and Occupancy to Facilitate Improved Management and Conservation

IF 4.6 2区 环境科学与生态学 Q1 BIODIVERSITY CONSERVATION
Mark D. Johnson, Sasha J. Tetzlaff, Aron D. Katz, Jeremy Tiemann, Chase Smith, Catherine E. Dana, Sarah E. Pearce, Mark A. Davis, Jordan H. Hartman, Jinelle H. Sperry
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引用次数: 0

Abstract

Aim

Freshwater mussels are considered among the most at-risk taxa in the world. As such, comprehensive monitoring assessments of what abiotic and biotic factors influence mussel occupancy will be vital for guiding effective conservation. Here, we analysed vertebrate and mussel environmental DNA (eDNA) metabarcoding data to explore the influence of biotic (i.e., host fish diversity, predator presence, and community composition) and abiotic (i.e., drainage size, forest cover, and stream order) factors on freshwater mussel populations.

Location

This study utilised water samples and tactile survey data collected from streams throughout Fort Johnson, Louisiana.

Methods

We first evaluated the effectiveness of eDNA metabarcoding for characterising freshwater communities based on previous conventional tactile surveys. Next, we used eDNA metabarcoding analysis for freshwater mussels and vertebrate species alongside GIS-derived satellite remote sensing data to assess how various biotic and abiotic variables impact freshwater mussel eDNA occupancy.

Results

Our eDNA metabarcoding survey largely agreed with both historical and contemporary surveys on Fort Johnson, while uniquely detecting Louisiana pigtoe (Pleurobema riddellii), a proposed threatened species under the US Endangered Species Act. We also found that eDNA detections and occupancy had strong seasonal variation, with increased read abundance and diversity in the spring. Vertebrate, fish, and predator diversity (as a function of habitat quality) were strongly predictive of mussel occupancy, supporting the concept of land managers focusing on the entire ecosystem for mussel conservation. Lastly, we found that percent forest cover and drainage basin size influenced mussel eDNA occupancy, informing habitat associations for mussel species of interest (i.e., the mussels occupied larger drainage sizes and perennial streams).

Conclusions

Our results demonstrate that combining eDNA metabarcoding of target and non-target species with occupancy modelling can provide insights into the ecology of freshwater mussels and is a useful tool to improve their conservation and management.

Abstract Image

环境DNA元条形码阐明淡水贻贝的多样性和占用,以促进改善管理和保护
淡水贻贝被认为是世界上最濒危的物种之一。因此,对影响贻贝占用的非生物和生物因素进行综合监测评估对于指导有效保护贻贝至关重要。在此,我们分析了脊椎动物和贻贝环境DNA (eDNA)元条形码数据,以探索生物(即宿主鱼类多样性、捕食者存在和群落组成)和非生物(即排水大小、森林覆盖和溪流顺序)因素对淡水贻贝种群的影响。这项研究利用了从路易斯安那州约翰逊堡的溪流中收集的水样和触觉调查数据。方法基于以往的常规触觉调查,我们首先评估了eDNA元条形码在表征淡水群落方面的有效性。接下来,我们使用淡水贻贝和脊椎动物物种的eDNA元条形码分析以及gis衍生的卫星遥感数据来评估各种生物和非生物变量如何影响淡水贻贝的eDNA占用。结果我们的eDNA元条形码调查结果与Fort Johnson的历史和当代调查结果基本一致,并独特地检测到美国濒危物种法案下的濒危物种路易斯安那猪脚趾(Pleurobema riddellii)。我们还发现,eDNA的检测和占用具有强烈的季节变化,在春季读取丰度和多样性增加。脊椎动物、鱼类和捕食者多样性(作为栖息地质量的函数)对贻贝的占用有很强的预测作用,这支持了土地管理者关注整个生态系统以保护贻贝的概念。最后,我们发现森林覆盖率百分比和流域大小影响贻贝的eDNA占用,为贻贝物种的栖息地关联提供信息(即贻贝占据更大的流域和多年生溪流)。结论将目标物种和非目标物种的eDNA元条形码与占用模型相结合,可以深入了解淡水贻贝的生态,是改善贻贝保护和管理的有效工具。
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来源期刊
Diversity and Distributions
Diversity and Distributions 环境科学-生态学
CiteScore
8.90
自引率
4.30%
发文量
195
审稿时长
8-16 weeks
期刊介绍: Diversity and Distributions is a journal of conservation biogeography. We publish papers that deal with the application of biogeographical principles, theories, and analyses (being those concerned with the distributional dynamics of taxa and assemblages) to problems concerning the conservation of biodiversity. We no longer consider papers the sole aim of which is to describe or analyze patterns of biodiversity or to elucidate processes that generate biodiversity.
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