Sorption of copper and NO synthase effectors on hydroxyapatite and effect of bovine serum albumin on the sorption process

Abstract

The sequential and combined sorption of bovine serum albumin (BSA) and copper ions on nanohydroxyapatite (HAP) changes the sorption capacity and sorption mechanism and affects the sorption reaction constants. Ligands, effectors of NO synthase N-(5,6-dihydro-4H-1,3-thiazin-2-yl)benzamide (iNOS inhibitor, L¹) and N-(4-isopropylphenyl)-N-(1-imminoethyl)piperidine-1-carbothioamide hydrobromide (NOS activator, L²), are not sorbed on HAP. However, the CuL¹₂Cl₂ complex shows weak binding to HAP possibly due to a higher sorption capacity of copper ions, which can displace calcium ions.