Targeted bio-imaging in discriminating normal and cancerous cells using dual-doped carbon dots derived from Dahlia pinnata flower extract

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-03-25 DOI:10.1007/s00604-025-07100-5

C. Dhanush, M. Ismayati, M. G. Sethuraman

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引用次数: 0

Abstract

This research delves into utilizing dual-doped (N & S) carbon dots (DDCDs) sourced from Dahlia pinnata flower extract for targeted bio-imaging, aiming to differentiate between normal and cancerous cells. The synthesized DDCDs, incorporating nitrogen and sulfur, exhibited unique optical, structural, and morphological properties with an average size of about 3.25 nm. These DDCDs demonstrate strong fluorescence and display excitation-dependent emission behavior, as confirmed by the results of photoluminescence spectroscopy. Folic acid conjugation with DDCDs enhances their specificity towards cancer cells expressing the folate receptor. Through comprehensive characterization, the study demonstrates the successful synthesis and functionalization of these DDCDs. Even at a concentration of 200 µg/ml, these DDCDs demonstrated low cytotoxicity. In vitro experiments on both normal and cancer cell lines reveal distinct fluorescence responses, showcasing the potential of these bio-compatible DDCDs for precise bio-imaging in cancer diagnostics. This work opens avenues for utilizing natural sources in nanomaterial synthesis for biomedical applications, contributing to the advancement of targeted cellular imaging technologies.

Graphical Abstract

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这项研究利用从大丽花提取物中提取的双掺杂（N & S）碳点（DDCDs）进行靶向生物成像，旨在区分正常细胞和癌细胞。合成的 DDCDs 含有氮和硫，具有独特的光学、结构和形态特性，平均尺寸约为 3.25 纳米。光致发光光谱的结果证实，这些 DDCDs 具有很强的荧光和随激发而发射的特性。叶酸与 DDCDs 的共轭作用增强了它们对表达叶酸受体的癌细胞的特异性。通过综合表征，该研究证明了这些 DDCDs 的成功合成和功能化。即使在 200 µg/ml 的浓度下，这些 DDCDs 的细胞毒性也很低。对正常细胞系和癌细胞系进行的体外实验显示了不同的荧光反应，展示了这些生物兼容的 DDCDs 在癌症诊断的精确生物成像方面的潜力。这项工作开辟了利用天然资源合成纳米材料用于生物医学应用的途径，促进了靶向细胞成像技术的发展。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.