Comparison of different graft materials on the proliferation, mineralization and mineralized tissue-related gene expressions of cementoblasts

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine Pub Date : 2025-03-26 DOI:10.1007/s10856-024-06840-9

E. Elif Mutafcilar Velioğlu, S. Buket Bozkurt, Werner Götz, Sema S. Hakki

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引用次数: 0

Abstract

Cementoblasts and formation of new cementum are crucial for periodontal regeneration. The aim of this study is to investigate the effect of different graft materials on cementoblast’s proliferation, mineralization and mineralized tissue-related gene expressions in-vitro. Immortalized mouse cementoblasts (OCCM-30) were treated with the media containing released components of graft materials (100 mg/ml ratio; waited in 5% FBS containing media for 3 days). Proliferation of the cells was evaluated using a real-time cell analyzer for 170 h and wound healing assay was performed to determine the migration of OCCM-30 cells. Total RNA was isolated on days 3 and 6, and biomineralization of the cementoblasts was assessed using von Kossa staining. mRNA expressions of bone sialoprotein (BSP), osteocalcin (OCN), collagen type I (COL-I), runt-related transcription factor2 (Runx2), and alkaline phosphatase (ALP) genes were examined using quantitative RT-PCR. While there was no significant difference at 72 h, all test groups showed significant reduction of cementoblast’s proliferation at 96^th and 120^th h. All graft materials increased cell migration to the experimental wounded area. While Bio-Oss^® showed significantly better effects on all mineralized tissue associated gene mRNA expressions on day 3 (p < 0.01), Nanobone^® upregulated Runx2 (p < 0.01) and BSP (p < 0.05), Emdogain^® induced OCN (p < 0.05) and Runx2 (p < 0.05). Endobon^® upregulated Runx2 only on day 3 and 6 (p < 0.05). The most prominent increase in mineralized nodule formation was observed in Nanobone^® (p < 0.05). In conclusion, released components of all graft materials have positive effects on the cementoblast’s functions while Bio-Oss^® might be preferable for gene expressions. Nanobone^® has superiority for the biomineralization of the cementoblasts. The interaction of the graft materials and the cementoblasts is critical for the formation of new cementum required for periodontal regeneration.

Graphical Abstract

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牙骨质母细胞和新牙骨质的形成对牙周再生至关重要。本研究旨在探讨不同移植材料对骨水泥母细胞增殖、矿化和矿化组织相关基因表达的体外影响。用含有移植物材料释放成分的培养基（比例为 100 毫克/毫升；在含 5% FBS 的培养基中等待 3 天）处理永生化小鼠骨水泥母细胞（OCCM-30）。使用实时细胞分析仪评估细胞增殖 170 小时，并进行伤口愈合试验以确定 OCCM-30 细胞的迁移情况。在第 3 天和第 6 天分离了总 RNA，并使用 von Kossa 染色法评估了骨水泥母细胞的生物矿化。虽然在 72 小时内没有明显差异，但在第 96 和 120 小时内，所有试验组的水门汀母细胞增殖均明显减少。在第 3 天，Bio-Oss® 对所有矿化组织相关基因 mRNA 表达的影响明显更好（p < 0.01），Nanobone® 上调 Runx2（p < 0.01）和 BSP（p < 0.05），Emdogain® 诱导 OCN（p < 0.05）和 Runx2（p < 0.05）。Endobon® 仅在第 3 天和第 6 天调控 Runx2（p < 0.05）。在 Nanobone® 中，矿化结核形成的增加最为显著（p < 0.05）。总之，所有移植材料释放的成分对骨水泥母细胞的功能都有积极影响，而 Bio-Oss® 在基因表达方面可能更胜一筹。Nanobone® 在骨水泥母细胞的生物矿化方面更具优势。移植材料和骨水泥母细胞的相互作用对于牙周再生所需的新骨水泥的形成至关重要。

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.