Latest advances in research methods for high-yield cellulase production

Abstract

Lignocellulosic biomass, due to its accessibility, abundance, and environmental friendliness, has become a promising renewable resource. The key role of cellulases in the degradation of lignocellulosic biomass has been extensively studied and plays a critical role in various industrial applications. However, the high production costs, low enzymatic activity, and poor stability of cellulases limit their application. Consequently, an increasing number of studies are dedicated to enhancing the activity and stability of cellulases to achieve efficient and economical degradation and utilization of lignocellulosic biomass. This review comprehensively analyzes some methods currently used to enhance cellulase production and the latest research advancements. It mainly introduces high-yield cellulase strain screening, selection and breeding of high-yield cellulase-producing strains, molecular modification of cellulases, and cellulase immobilization. It discusses the shortcomings of these methods and the latest research progress, providing references for further improving the performance of cellulases in the future.