Effect of fermentation conditions on bioactive compounds, physicochemical properties, antimicrobial activities, and cellulosic pellicle formation in black tea Kombucha

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2025-03-05 DOI:10.1016/j.bcab.2025.103547

Boying Wang , Kay Rutherfurd-Markwick , Xue-Xian Zhang , Cheng Xu , Anthony N. Mutukumira

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Abstract

This study aimed to determine the effect of various fermentation factors on levels of bioactive components, antioxidants, and antimicrobial activities of black tea Kombucha prepared with a starter culture obtained in New Zealand. Of the seven measured phenolic compounds, caffeine, gallic acid and theobromine were the predominant ones in the 17 fermented Kombucha samples developed using the Plackett-Burman design. All samples exhibited antioxidant activities for scavenging DPPH (20.78–91.28%) and ABTS+ (22.63–551.10 μg TE/mL). The phenolic content and antioxidant activities were significantly influenced by the concentrations of black tea used (p < 0.05). Kombucha samples with the highest titratable acidity (1.03%) exhibited the strongest antimicrobial activities against Staphylococcus aureus MCTIC 4163, Bacillus cereus MU-A44 and Escherichia coli NCTIC 8196. Notably, the three Kombucha samples K15, K11 and K8 produced in this study contained high levels of bioactive compounds and exhibited strong antioxidant and antimicrobial activities.

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发酵条件对康普茶生物活性化合物、理化性质、抗菌活性和纤维素膜形成的影响

本研究旨在确定各种发酵因素对用新西兰获得的起始培养物制备的红茶昆布茶的生物活性成分、抗氧化剂和抗菌活性水平的影响。在采用 "普拉克特-伯曼 "设计法制备的 17 个发酵康普茶样品中，测得的七种酚类化合物中以咖啡因、没食子酸和可可碱为主。所有样品都具有清除 DPPH（20.78-91.28%）和 ABTS+（22.63-551.10 μg TE/mL）的抗氧化活性。酚含量和抗氧化活性受所用红茶浓度的显著影响（p < 0.05）。滴定酸度最高（1.03%）的昆布茶样品对金黄色葡萄球菌 MCTIC 4163、蜡样芽孢杆菌 MU-A44 和大肠杆菌 NCTIC 8196 的抗菌活性最强。值得注意的是，本研究中生产的三种 Kombucha 样品 K15、K11 和 K8 含有大量生物活性化合物，具有很强的抗氧化和抗菌活性。

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来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.