{"title":"Pod lignin biosynthesis contributes to pre-harvest sprouting tolerance of rapeseed","authors":"Tianhua Chen, Qing’ao Cai, Caili Liu, Rui Li, Liyan Wang, Jian’an Chen, Nian Liu, Boshi Yang, Shuo Zhou, Zonghe Zhu, Kejin Zhou, Fugui Zhang","doi":"10.1016/j.envexpbot.2025.106129","DOIUrl":null,"url":null,"abstract":"<div><div>Pre-harvest sprouting (PHS) poses a major hazard to rapeseed (<em>Brassica napus</em> L.) production, particularly under rainy, moist conditions during harvest season. PHS tolerance mainly depends on seed dormancy and pericarp structure in other crops. However, little was known about the mechanisms underlying PHS tolerance in rapeseed. In this study, an elite PHS-tolerant genotype was screened from 750 global rapeseed germplasm resources by pod imbibition and seed germination assays. Results of imbibition dynamics have shown that the water absorption was slower in pod shell and seeds within pod of the PHS-tolerant genotype. The tolerant genotype also had higher pod shell thickness, cellulose content, and lignin content. The pod shell of the tolerant genotype was significantly enriched in a large number of differential metabolites involved in the phenylpropanoid metabolism pathway, which contribute to lignin biosynthesis. Moreover, lignin synthesis related genes <em>BnaPAL4</em>, <em>Bna4CL1</em>, <em>BnaCCR1</em>, <em>BnaHST</em> and <em>BnaPER42</em> significantly more expressed in the PHS-tolerant genotype than in the PHS-sensitive genotype. More lignin accumulation in the pod could protect rapeseed from PHS by decreasing the pericarp permeability. All these findings could provide valuable genetic resources for the breeding of PHS-tolerant rapeseed cultivars and understanding of PHS mechanism in <em>Brassica</em> species.</div></div>","PeriodicalId":11758,"journal":{"name":"Environmental and Experimental Botany","volume":"232 ","pages":"Article 106129"},"PeriodicalIF":4.5000,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental and Experimental Botany","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0098847225000462","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Pre-harvest sprouting (PHS) poses a major hazard to rapeseed (Brassica napus L.) production, particularly under rainy, moist conditions during harvest season. PHS tolerance mainly depends on seed dormancy and pericarp structure in other crops. However, little was known about the mechanisms underlying PHS tolerance in rapeseed. In this study, an elite PHS-tolerant genotype was screened from 750 global rapeseed germplasm resources by pod imbibition and seed germination assays. Results of imbibition dynamics have shown that the water absorption was slower in pod shell and seeds within pod of the PHS-tolerant genotype. The tolerant genotype also had higher pod shell thickness, cellulose content, and lignin content. The pod shell of the tolerant genotype was significantly enriched in a large number of differential metabolites involved in the phenylpropanoid metabolism pathway, which contribute to lignin biosynthesis. Moreover, lignin synthesis related genes BnaPAL4, Bna4CL1, BnaCCR1, BnaHST and BnaPER42 significantly more expressed in the PHS-tolerant genotype than in the PHS-sensitive genotype. More lignin accumulation in the pod could protect rapeseed from PHS by decreasing the pericarp permeability. All these findings could provide valuable genetic resources for the breeding of PHS-tolerant rapeseed cultivars and understanding of PHS mechanism in Brassica species.
期刊介绍:
Environmental and Experimental Botany (EEB) publishes research papers on the physical, chemical, biological, molecular mechanisms and processes involved in the responses of plants to their environment.
In addition to research papers, the journal includes review articles. Submission is in agreement with the Editors-in-Chief.
The Journal also publishes special issues which are built by invited guest editors and are related to the main themes of EEB.
The areas covered by the Journal include:
(1) Responses of plants to heavy metals and pollutants
(2) Plant/water interactions (salinity, drought, flooding)
(3) Responses of plants to radiations ranging from UV-B to infrared
(4) Plant/atmosphere relations (ozone, CO2 , temperature)
(5) Global change impacts on plant ecophysiology
(6) Biotic interactions involving environmental factors.