Pod lignin biosynthesis contributes to pre-harvest sprouting tolerance of rapeseed

IF 4.5 2区生物学 Q2 ENVIRONMENTAL SCIENCES

Environmental and Experimental Botany Pub Date : 2025-03-22 DOI:10.1016/j.envexpbot.2025.106129

Tianhua Chen, Qing’ao Cai, Caili Liu, Rui Li, Liyan Wang, Jian’an Chen, Nian Liu, Boshi Yang, Shuo Zhou, Zonghe Zhu, Kejin Zhou, Fugui Zhang

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引用次数: 0

Abstract

Pre-harvest sprouting (PHS) poses a major hazard to rapeseed (Brassica napus L.) production, particularly under rainy, moist conditions during harvest season. PHS tolerance mainly depends on seed dormancy and pericarp structure in other crops. However, little was known about the mechanisms underlying PHS tolerance in rapeseed. In this study, an elite PHS-tolerant genotype was screened from 750 global rapeseed germplasm resources by pod imbibition and seed germination assays. Results of imbibition dynamics have shown that the water absorption was slower in pod shell and seeds within pod of the PHS-tolerant genotype. The tolerant genotype also had higher pod shell thickness, cellulose content, and lignin content. The pod shell of the tolerant genotype was significantly enriched in a large number of differential metabolites involved in the phenylpropanoid metabolism pathway, which contribute to lignin biosynthesis. Moreover, lignin synthesis related genes BnaPAL4, Bna4CL1, BnaCCR1, BnaHST and BnaPER42 significantly more expressed in the PHS-tolerant genotype than in the PHS-sensitive genotype. More lignin accumulation in the pod could protect rapeseed from PHS by decreasing the pericarp permeability. All these findings could provide valuable genetic resources for the breeding of PHS-tolerant rapeseed cultivars and understanding of PHS mechanism in Brassica species.

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豆荚木质素生物合成对油菜籽采前发芽耐性的影响

收获前发芽（PHS）是油菜（Brassica napus L.）生产的主要危害，特别是在收获季节多雨、潮湿的条件下。其他作物对小灵通的耐受性主要取决于种子休眠和果皮结构。然而，对油菜籽耐小灵通的机制知之甚少。本研究通过荚果吸吸和种子萌发试验，从全球750个油菜种质资源中筛选了一个耐ps的优良基因型。吸胀动力学结果表明，耐phs基因型荚果壳内和荚果内的吸水速度较慢。耐受性基因型荚果壳厚度、纤维素含量和木质素含量也较高。耐受性基因型荚果壳显著富集了大量参与苯丙素代谢途径的差异代谢物，这些代谢物有助于木质素的生物合成。此外，木质素合成相关基因BnaPAL4、Bna4CL1、BnaCCR1、BnaHST和BnaPER42在ps耐受基因型中的表达量显著高于ps敏感基因型。荚果中木质素的积累可以通过降低果皮通透性来保护油菜籽免受PHS的侵害。这些研究结果可为选育耐PHS的油菜品种和了解芸苔属植物的PHS机制提供宝贵的遗传资源。

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来源期刊

Environmental and Experimental Botany 环境科学-环境科学

CiteScore

9.30

自引率

5.30%

发文量

342

审稿时长

26 days

期刊介绍： Environmental and Experimental Botany (EEB) publishes research papers on the physical, chemical, biological, molecular mechanisms and processes involved in the responses of plants to their environment. In addition to research papers, the journal includes review articles. Submission is in agreement with the Editors-in-Chief. The Journal also publishes special issues which are built by invited guest editors and are related to the main themes of EEB. The areas covered by the Journal include: (1) Responses of plants to heavy metals and pollutants (2) Plant/water interactions (salinity, drought, flooding) (3) Responses of plants to radiations ranging from UV-B to infrared (4) Plant/atmosphere relations (ozone, CO2 , temperature) (5) Global change impacts on plant ecophysiology (6) Biotic interactions involving environmental factors.