Integrated metabolomic and transcriptomic analyses reveal anthocyanin biosynthesis mechanisms and the regulatory role of LjAN2 in Lonicera japonica

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-03-22 DOI:10.1016/j.plaphy.2025.109824

Zhengwei Tan , Dandan Lu , Lei Li , Yongliang Yu , Xiaoyu Su , Yao Sun , Yiwen Cao , Chunming Li , Wei Dong , Hongqi Yang , Qing Yang , Sufang An , Huizhen Liang

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引用次数: 0

Abstract

Lonicera japonica flowers are a very commonly used traditional Chinese herb. Anthocyanins are the source of flower pigments, and also are renowned for their therapeutic activities. However, the specific anthocyanin composition and regulatory mechanisms governing their accumulation in L. japonica varieties remain unclear. Here, we first investigated the changes in flower color and anthocyanin content during development in the green flower (GFLJ) and the purple flower (PFLJ) cultivars of L. japonica. Results show GFLJ has green flowers and PFLJ has purple flowers, which are especially remarkable during S3-S4 stages. Accordingly, PFLJ had much higher (>10 times) anthocyanins contents at all the six flower stages than those of GFLJ. Further metabolomic analysis in S3 stage flowers found that most anthocyanins showed increased accumulation, whereas flavones and flavonols showed decreased accumulation in PFLJ compared to GFLJ. Transcriptome analysis identified 21 (85.7 % upregulated) anthocyanin synthase gene DEGs, and 23 MYB transcription factor (TF) DEGs (play essential roles in regulating anthocyanin biosynthesis). In addition, 19 GST and 14 MATE DEGs (play key roles in anthocyanins accumulation) were identified. Further, we found a novel MYB TF (LjAN2) that showed much higher expression in PFLJ. LjAN2 overexpression in tobacco led to purple leaves, and the upregulation of anthocyanin synthase genes (NtCHS and NtANS), as well as increased anthocyanin accumulation. This research offers a comprehensive understanding of the molecular basis of anthocyanin biosynthesis in L. japonica, highlighting its potential applications in the pharmaceutical industry.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.