Exploring associations among pro-inflammatory cytokines, osteoarthritis, and gut microbiome composition in individuals with obesity using machine learning

Cameron Kurz , Liubov Arbeeva , M. Andrea Azcarate-Peril , Delisha A. Stewart , B. Duncan X. Lascelles , Richard F. Loeser , Amanda E. Nelson
{"title":"Exploring associations among pro-inflammatory cytokines, osteoarthritis, and gut microbiome composition in individuals with obesity using machine learning","authors":"Cameron Kurz ,&nbsp;Liubov Arbeeva ,&nbsp;M. Andrea Azcarate-Peril ,&nbsp;Delisha A. Stewart ,&nbsp;B. Duncan X. Lascelles ,&nbsp;Richard F. Loeser ,&nbsp;Amanda E. Nelson","doi":"10.1016/j.ocarto.2025.100603","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>To investigate potentially novel and modifiable mechanisms of the effects of gut microbiome composition on obesity-related osteoarthritis (OA), focusing on cross-sectional relationships between microbiota, cytokines, and lipopolysaccharide (LPS).</div></div><div><h3>Design</h3><div>Johnston County OA Project participants (n ​= ​64) with (cases) and without (controls) OA in hands and knees, with age ≥55 years and obesity (BMI ≥30 ​kg/m<sup>2</sup>), provided samples for multiplex cytokine, LPS, and fecal microbiota analysis. Latent Dirichlet Allocation (LDA), a machine learning method to detect latent groups within data, was used to identify microbial enterotypes. LDA regression models were used to evaluate associations of enterotypes with demographics, cytokines associated with OA, and LPS.</div></div><div><h3>Results</h3><div>We identified 5 enterotypes. Enterotypes 3, 4 (most prevalent in our sample), and 5, dominated respectively by genera <em>Akkermansia</em>, <em>Bacteroides</em>, <em>Ruminococcus</em>/<em>Phascolarctobacterium</em>, were positively associated with control status, and inversely associated with levels of at least two cytokines associated with OA in our sample. We observed no associations of enterotypes with LPS levels. Enterotype 3 was inversely associated with thrombopoietin and IL-4 levels (b [95 ​% CIs] −0.19 [−0.43, 0.05] and −0.17 [−0.42, 0.08]), enterotype 5 with osteopontin and thrombopoietin (−0.23 [−0.49, 0.03] and −0.24 [−0.51, 0.04]), and enterotype 4 was inversely associated with all 3 of these cytokines (b −0.20 to −0.35).</div></div><div><h3>Conclusion</h3><div>Three of five identified enterotypes were inversely associated with OA status and levels of OA associated cytokines. These exploratory analyses revealed associations between the gut microbiome, cytokines, and OA outcomes, suggesting potentially cytokine-mediated mechanisms of the effects of gut composition on OA in obese individuals, and providing a basis for further investigation of the underlying causal mechanisms.</div></div>","PeriodicalId":74377,"journal":{"name":"Osteoarthritis and cartilage open","volume":"7 2","pages":"Article 100603"},"PeriodicalIF":0.0000,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Osteoarthritis and cartilage open","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2665913125000391","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Objective

To investigate potentially novel and modifiable mechanisms of the effects of gut microbiome composition on obesity-related osteoarthritis (OA), focusing on cross-sectional relationships between microbiota, cytokines, and lipopolysaccharide (LPS).

Design

Johnston County OA Project participants (n ​= ​64) with (cases) and without (controls) OA in hands and knees, with age ≥55 years and obesity (BMI ≥30 ​kg/m2), provided samples for multiplex cytokine, LPS, and fecal microbiota analysis. Latent Dirichlet Allocation (LDA), a machine learning method to detect latent groups within data, was used to identify microbial enterotypes. LDA regression models were used to evaluate associations of enterotypes with demographics, cytokines associated with OA, and LPS.

Results

We identified 5 enterotypes. Enterotypes 3, 4 (most prevalent in our sample), and 5, dominated respectively by genera Akkermansia, Bacteroides, Ruminococcus/Phascolarctobacterium, were positively associated with control status, and inversely associated with levels of at least two cytokines associated with OA in our sample. We observed no associations of enterotypes with LPS levels. Enterotype 3 was inversely associated with thrombopoietin and IL-4 levels (b [95 ​% CIs] −0.19 [−0.43, 0.05] and −0.17 [−0.42, 0.08]), enterotype 5 with osteopontin and thrombopoietin (−0.23 [−0.49, 0.03] and −0.24 [−0.51, 0.04]), and enterotype 4 was inversely associated with all 3 of these cytokines (b −0.20 to −0.35).

Conclusion

Three of five identified enterotypes were inversely associated with OA status and levels of OA associated cytokines. These exploratory analyses revealed associations between the gut microbiome, cytokines, and OA outcomes, suggesting potentially cytokine-mediated mechanisms of the effects of gut composition on OA in obese individuals, and providing a basis for further investigation of the underlying causal mechanisms.
利用机器学习探索肥胖症患者体内促炎细胞因子、骨关节炎和肠道微生物组组成之间的关系
目的探讨肠道微生物群组成对肥胖相关性骨关节炎(OA)影响的潜在新颖和可改变的机制,重点研究微生物群、细胞因子和脂多糖(LPS)之间的横断面关系。约翰斯顿县OA项目参与者(n = 64)有(例)和没有(对照组)手和膝盖OA,年龄≥55岁,肥胖(BMI≥30 kg/m2),提供样品进行多重细胞因子、LPS和粪便微生物群分析。潜在狄利克雷分配(Latent Dirichlet Allocation, LDA)是一种检测数据中潜在群体的机器学习方法,用于鉴定微生物肠型。使用LDA回归模型评估肠型与人口统计学、与OA相关的细胞因子和LPS的关系。结果共鉴定出5种肠型。3型、4型(在我们的样本中最普遍)和5型(分别由Akkermansia属、拟杆菌属、Ruminococcus/Phascolarctobacterium属主导)与对照状态呈正相关,与我们样本中至少两种与OA相关的细胞因子水平呈负相关。我们没有观察到肠型与LPS水平的关联。肠型3与血小板生成素和IL-4水平呈负相关(b [95% ci] - 0.19[- 0.43, 0.05]和- 0.17[- 0.42,0.08]),肠型5与骨桥蛋白和血小板生成素水平呈负相关(- 0.23[- 0.49,0.03]和- 0.24[- 0.51,0.04]),肠型4与所有这3种细胞因子呈负相关(b - 0.20至- 0.35)。结论鉴定的5种肠型中有3种与OA状态和OA相关细胞因子水平呈负相关。这些探索性分析揭示了肠道微生物组、细胞因子和OA结果之间的关联,提示了肠道成分对肥胖个体OA影响的潜在细胞因子介导机制,并为进一步研究潜在的因果机制提供了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Osteoarthritis and cartilage open
Osteoarthritis and cartilage open Orthopedics, Sports Medicine and Rehabilitation
CiteScore
3.30
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信