A study on the effect of the number of expansion units in a microfluidic chip on hyaluronidase-free oocyte denudation in mammals.

Q3 Biochemistry, Genetics and Molecular Biology
Journal of Electrical Bioimpedance Pub Date : 2025-03-20 eCollection Date: 2025-01-01 DOI:10.2478/joeb-2025-0004
Ashraf Hisham Dessouky, Haitham El-Hussieny, Taymour Mohammed El-Sherry, Victor Parque, Ahmed M R Fath El-Bab
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Abstract

In Vitro Fertilization (IVF) and Intracytoplasmic Sperm Injection (ICSI) are well-known fertility treatments that, due to resource-intensive, high degree of expertise required, and frequent subpar performances, often yield in high costs for treatment cycles. Microfluidic technology has enabled cost-effective egg-handling procedures towards new assistive reproductive devices: oocytes are subjected to microchannels with jagged surfaces to let shear stress remove undesirable cumulus cells, and microchannels with expansion units facilitate the transport of oocytes in chips. However, although the previous works have studied the influence of shear stress on oocyte denudation and the role of microchannel teeth in optimizing cell handling efficiency, the study of configurations of jagged surfaces and expansion units in microfluidic devices has remained elusive. Also, comprehensive analysis using both computational fluid dynamics (CFD) and real-world microfluidic devices has remained an unexplored area. To fill the abovementioned gap, this paper studies microfluidics chips with different expansion units to depict the behavior of oocytes when subjected to controlled input flows. The proposed chips were developed and fabricated using a direct engraving CO2 laser machine on polymethyl methacrylate (PMMA) sheets and bonded in a natural ventilation lab oven, rendering the highly efficient and low-cost microfluidic chips for oocyte denudation. The effect of the expansion units has been investigated in CFD simulation and real lab experimentation with mature buffalo oocytes at a constant flow rate, and a chip with five expansion units arranged in two lines achieved 98.33% denudation efficiency, low-cost fabrication (about 1 USD), and quick fabrication time (about 20 minutes).

微流控芯片中膨胀单元数目对哺乳动物无透明质酸酶卵母细胞剥落影响的研究。
体外受精(IVF)和胞浆内单精子注射(ICSI)是众所周知的生育治疗方法,由于资源密集,需要高度的专业知识,并且经常表现不佳,通常导致治疗周期的高成本。微流控技术为新的辅助生殖装置提供了具有成本效益的卵子处理程序:卵母细胞置于锯齿状表面的微通道中,让剪切应力去除不需要的积云细胞,而带有膨胀单元的微通道有助于卵母细胞在芯片中的运输。然而,尽管之前的工作已经研究了剪切应力对卵母细胞剥蚀的影响以及微通道齿在优化细胞处理效率中的作用,但对微流体装置中锯齿状表面和膨胀单元的配置的研究仍然是难以实现的。此外,利用计算流体动力学(CFD)和现实世界的微流体装置进行综合分析仍然是一个未开发的领域。为了填补上述空白,本文研究了具有不同扩展单元的微流体芯片,以描述卵母细胞在受控输入流下的行为。该芯片采用直接雕刻CO2激光机在聚甲基丙烯酸甲酯(PMMA)薄片上开发和制造,并在自然通风的实验室烘箱中粘合,实现了用于卵母细胞剥落的高效低成本微流控芯片。利用成熟水牛卵母细胞在恒定流速下的CFD模拟和真实实验室实验研究了膨胀单元的效果,结果表明,两行排列5个膨胀单元的芯片的剥落效率为98.33%,制造成本低(约1美元),制造时间短(约20分钟)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Electrical Bioimpedance
Journal of Electrical Bioimpedance Engineering-Biomedical Engineering
CiteScore
3.00
自引率
0.00%
发文量
8
审稿时长
17 weeks
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