Bacterial membrane vesicles restore gut anaerobiosis.

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

npj Biofilms and Microbiomes Pub Date : 2025-03-22 DOI:10.1038/s41522-025-00676-z

Norman Pitt, Madeleine Morrissette, Michael F Gates, Rachel Bargabos, Megan Krumpoch, Bryson Hawkins, Kim Lewis

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Abstract

Inflammation damages the epithelial cell barrier, allowing oxygen to leak into the lumen of the gut. Respiring E. coli and other Enterobacteriaceae produce proinflammatory lipopolysaccharide, exacerbating inflammatory bowel disease. Here we show that respiring membrane vesicles (MV) from E. coli ameliorate symptoms in a mouse model of gut inflammation. Membrane vesicle treatment diminished weight loss and limited shortening of the colon. Notably, oxygenation of the colonic epithelium was significantly decreased in animals receiving wild type MVs, but not MVs from an E. coli mutant lacking cytochromes. Metatranscriptomic analysis of the microbiome shows an increase in anaerobic Lactobacillaceae and a decrease in Enterobacteriaceae, as well as a general shift towards fermentation in MV-treated mice. This is accompanied by a decrease in proinflammatory TNF-α. We report that MVs may lead to the development of a novel type of a therapeutic for dysbiosis, and for treating IBD.

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细菌膜囊恢复肠道厌氧症。

炎症会破坏上皮细胞屏障，使氧气泄漏到肠道内腔。呼吸性大肠杆菌和其他肠杆菌科产生促炎脂多糖，加剧炎症性肠病。在这里，我们展示了大肠杆菌呼吸膜泡（MV）改善小鼠肠道炎症模型的症状。膜囊泡治疗减轻了体重减轻，限制了结肠缩短。值得注意的是，在接受野生型mv的动物中，结肠上皮的氧合明显减少，而来自缺乏细胞色素的大肠杆菌突变体的mv则没有。微生物组的超转录组学分析显示，在mv处理的小鼠中，厌氧乳酸杆菌科增加，肠杆菌科减少，以及向发酵的总体转变。这伴随着促炎TNF-α的减少。我们报道，MVs可能导致一种新型的治疗失调和治疗IBD的发展。

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来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.